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Nucleolin/C23 Recombinant antibody, PBS Only

Nucleolin/C23 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240029F8

Cat no : 83380-1-PBS

Print datasheet

Synonyms

C23, nucleolin, Protein C23, NCL, FLJ45706

验证数据展示

  • WB analysis of HeLa using 83380-1-RR (same clone as 83380-1-PBS)

    WB analysis of HeLa using 83380-1-RR (same clone as 83380-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83380-1-RR (Nucleolin/C23 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83380-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 83380-1-RR (same clone as 83380-1-PBS)

    IP experiment of HeLa using 83380-1-RR (same clone as 83380-1-PBS)

    IP result of anti-Nucleolin/C23 (IP:83380-1-RR, 4ug; Detection:83380-1-RR 1:5000) with HeLa cells lysate 1085 ug. This data was developed using the same antibody clone with 83380-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 83380-1-RR (same clone as 83380-1-PBS)

    IHC staining of human lung cancer using 83380-1-RR (same clone as 83380-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 83380-1-RR (Nucleolin/C23 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83380-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NCL antibody included
  • FC experiment of HEK-293 using 83380-1-RR (same clone as 83380-1-PBS)

    FC experiment of HEK-293 using 83380-1-RR (same clone as 83380-1-PBS)

    1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug Nucleolin/C23 Recombinant antibody (83380-1-RR, Clone:240029F8) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83380-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 83380-1-RR (same clone as 83380-1-PBS)

    IF Staining of HepG2 using 83380-1-RR (same clone as 83380-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Nucleolin/C23 antibody (83380-1-RR, Clone: 240029F8 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83380-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83380-1-RR

    Affinity and Kinetic Characterization of 83380-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83380-1-RR against Human Nucleolin/C23 were performed. The affinity constant is 2.47 nM.

Planning an IHC experiment? We recommend our IHCeasy NCL Ready-To-Use IHC Kit. NCL primary antibody included.

产品信息

The immunogen of 83380-1-PBS is Nucleolin/C23 Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA Application Description
Tested Reactivity human
Immunogen Nucleolin/C23 fusion protein Ag0859 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name nucleolin
Synonyms C23, nucleolin, Protein C23, NCL, FLJ45706
Calculated Molecular Weight 76 kDa
Observed Molecular Weight 100-110 kDa
GenBank Accession NumberBC006494
Gene Symbol NCL
Gene ID (NCBI) 4691
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP19338
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Nucleolin, also known as C23, involved in the control of transcription of ribosomal RNA (rRNA) genes by RNA polymerase I, in nucleocytoplasmic transportation of ribosomal components, and in ribosome maturation and assembly. It associated with intranucleolar chromatin and pre-ribosomal particles, and induced chromatin decondensation by binding to histione H1. Also it has a role in the process of transcriptional elongation. Whilst mammalian nucleolin has a predicted molecular mass of approximately 77 kDa, the apparent molecular mass is between 100 and 110 kDa, and has been attributed to the amino acid composition of the N-terminal domain, which is highly phosphorylated. (PMID: 15925566)