Nucleolin/C23 Recombinant antibody
Nucleolin/C23 Recombinant Antibody for WB, IP, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human
Applications
WB, IP, IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
240029F8
验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, MCF-7 cells |
Positive IP detected in | HeLa cells |
Positive IF detected in | HepG2 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunofluorescence (IF) | IF : 1:125-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
The immunogen of 83380-1-RR is Nucleolin/C23 Fusion Protein expressed in E. coli.
Tested Applications | WB, IP, IF/ICC, ELISA Application Description |
Tested Reactivity | Human |
Immunogen | Nucleolin/C23 fusion protein Ag0859 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | nucleolin |
Synonyms | C23, FLJ45706, NCL, nucleolin, Protein C23 |
Calculated Molecular Weight | 76 kDa |
Observed Molecular Weight | 100-110 kDa |
GenBank Accession Number | BC006494 |
Gene Symbol | NCL |
Gene ID (NCBI) | 4691 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purfication |
UNIPROT ID | P19338 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for Nucleolin/C23 antibody 83380-1-RR | Download protocol |
IF protocol for Nucleolin/C23 antibody 83380-1-RR | Download protocol |
IP protocol for Nucleolin/C23 antibody 83380-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |