CoraLite® Plus 488-conjugated Nucleolin/C23 Recombinant antibody
Nucleolin/C23 Uni-rAbTM Recombinant Antibody for IF/ICC
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
240029F8
验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy NCL Ready-To-Use IHC Kit. NCL primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 83380-1-PBS
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
The immunogen of CL488-83380 is Nucleolin/C23 Fusion Protein expressed in E. coli.
| Tested Applications | IF/ICC Application Description |
| Tested Reactivity | human |
| Immunogen | Nucleolin/C23 fusion protein Ag0859 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | nucleolin |
| Synonyms | C23, nucleolin, Protein C23, NCL, FLJ45706 |
| Calculated Molecular Weight | 76 kDa |
| Observed Molecular Weight | 100-110 kDa |
| GenBank Accession Number | BC006494 |
| Gene Symbol | NCL |
| Gene ID (NCBI) | 4691 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P19338 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Nucleolin, also known as C23, involved in the control of transcription of ribosomal RNA (rRNA) genes by RNA polymerase I, in nucleocytoplasmic transportation of ribosomal components, and in ribosome maturation and assembly. It associated with intranucleolar chromatin and pre-ribosomal particles, and induced chromatin decondensation by binding to histione H1. Also it has a role in the process of transcriptional elongation. Whilst mammalian nucleolin has a predicted molecular mass of approximately 77 kDa, the apparent molecular mass is between 100 and 110 kDa, and has been attributed to the amino acid composition of the N-terminal domain, which is highly phosphorylated. (PMID: 15925566)
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 Nucleolin/C23 antibody CL488-83380 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |