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Kiz Polyclonal antibody

Kiz Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

IF/ICC, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 21177-1-AP

Print datasheet

Synonyms

C20orf19, Centrosomal protein kizuna, HT013, KIZ, NCRNA00153, non protein coding RNA 153, PLK1S1, Polo like kinase 1 substrate 1

验证数据展示

  • WB analysis of HeLa using 21177-1-AP

    WB analysis of HeLa using 21177-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 21177-1-AP (C20orf19 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 21177-1-AP

    IP experiment of HeLa using 21177-1-AP

    IP result of anti-Kiz (IP:21177-1-AP, 3ug; Detection:21177-1-AP 1:500) with HeLa cells lysate 2000ug.

  • IHC staining of human testis using 21177-1-AP

    IHC staining of human testis using 21177-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 21177-1-AP (C20orf19 antibody) at dilution of 1:100 (under 10x lens).

  • IF Staining of HeLa using 21177-1-AP

    IF Staining of HeLa using 21177-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Kiz antibody (21177-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Gamma Tubulin antibody (66320-1-Ig, Clone: 3F9H8, red).

经过测试的应用

Positive WB detected inHeLa cells, human brain tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells, hTERT-RPE1 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 2 publications below

产品信息

The immunogen of 21177-1-AP is Kiz Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, IHC, IP, WB, ELISA Application Description
Cited ApplicationsIF
Tested Reactivity human
Cited Reactivityhuman, mouse
Immunogen Kiz fusion protein Ag15454 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name non-protein coding RNA 153
Synonyms C20orf19, Centrosomal protein kizuna, HT013, KIZ, NCRNA00153, non protein coding RNA 153, PLK1S1, Polo like kinase 1 substrate 1
Calculated Molecular Weight 673 aa, 75 kDa
Observed Molecular Weight 90-100 kDa
GenBank Accession NumberBC045826
Gene Symbol NCRNA00153
Gene ID (NCBI) 55857
RRIDAB_10755292
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ2M2Z5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for Kiz antibody 21177-1-APDownload protocol
IHC protocol for Kiz antibody 21177-1-APDownload protocol
IF protocol for Kiz antibody 21177-1-APDownload protocol
IP protocol for Kiz antibody 21177-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Science

A liquid-like spindle domain promotes acentrosomal spindle assembly in mammalian oocytes.

Authors - Chun So
View Article
humanIF

Science

The mechanism of acentrosomal spindle assembly in human oocytes

Authors - Tianyu Wu
View Article