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验证数据展示

  • IF Staining of human breast cancer using CL594-10233

    IF Staining of human breast cancer using CL594-10233

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CoraLite®594 NDE1 antibody (CL594-10233) at dilution of 1:200.

  • IF Staining of human breast cancer using CL594-10233

    IF Staining of human breast cancer using CL594-10233

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CoraLite®594 NDE1 antibody (CL594-10233) at dilution of 1:200.

  • Featured Product
  • KD/KO验证

CoraLite®594-conjugated NDE1 Polyclonal antibody

NDE1 Polyclonal Antibody for IF-P
Cat No. CL594-10233

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF-P

别名

FLJ20101, HOM TES 87, NDE1, NUDE, NUDE1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive IF-P detected inhuman breast cancer tissue
For other applications, we recommend the unconjugated version of this antibody, 10233-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-10233 targets NDE1 in IF-P applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF-P Application Description
经测试反应性 human, mouse, rat
免疫原 NDE1 fusion protein Ag0336 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 nudE nuclear distribution gene E homolog 1 (A. nidulans)
别名 FLJ20101, HOM TES 87, NDE1, NUDE, NUDE1
计算分子量 335 aa, 38 kDa
观测分子量 40 kDa
GenBank蛋白编号BC001421
基因名称 NDE1
Gene ID (NCBI) 54820
RRIDAB_2919725
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NXR1
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NDE1 (NUDE /NUDE1) is a LIS1-interacting protein. A signaling pathway containing NDE1, LIS1, and cytoplasmic dynein is conserved for eukaryotic nuclear migration and has profound impact on neuronal migration during development of the central nervous system. LIS1 and dynein have been shown to be involved in mitosis and NDE1 participates in a subset of dynein function in M phase. NDE1 is specifically phosphorylated in M phase in its serine/threonine phosphorylation motifs, probably by Cdc2 and also Erk1 and Erk2. A fraction of NDEl binds to centrosomes strongly in interphase and is localized to mitotic spindles in early M phase. Phosphorylation of NDE1 also regulates the cell-cycle-dependent distribution, possibly by increasing its dissociation rate at the microtubule-organizing center. This antibody is a rabbit polyclonal antibody raised against a peptide mapping within human NDE1.

实验方案

Product Specific Protocols
IF protocol for CL594 NDE1 antibody CL594-10233Download protocol
Standard Protocols
Click here to view our Standard Protocols