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验证数据展示

  • IF Staining of OS-RC-2 using 66050-1-Ig (same clone as 66050-1-PBS)

    IF Staining of OS-RC-2 using 66050-1-Ig (same clone as 66050-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed OS-RC-2 cells using NDUFA4L2 antibody (66050-1-Ig, Clone: 1G1H10 ) at dilution of 1:1200 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 66050-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 66050-1-Ig (same clone as 66050-1-PBS)

    WB analysis of HeLa using 66050-1-Ig (same clone as 66050-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66050-1-Ig (NDUFA4L2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66050-1-PBS in a different storage buffer formulation.

  • IF Staining of HUVEC using 66050-1-Ig (same clone as 66050-1-PBS)

    IF Staining of HUVEC using 66050-1-Ig (same clone as 66050-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using NDUFA4L2 antibody (66050-1-Ig, Clone: 1G1H10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66050-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66050-1-Ig (same clone as 66050-1-PBS)

    IHC staining of human breast cancer using 66050-1-Ig (same clone as 66050-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66050-1-Ig (NDUFA4L2 Antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66050-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NDUFA4L2 antibody included
  • IHC staining of human breast cancer using 66050-1-Ig (same clone as 66050-1-PBS)

    IHC staining of human breast cancer using 66050-1-Ig (same clone as 66050-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66050-1-Ig (NDUFA4L2 Antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66050-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NDUFA4L2 antibody included
  • Cytometric bead array standard curve of MP50529-1

    Cytometric bead array standard curve of MP50529-1

    Cytometric bead array standard curve of MP50529-1, NDUFA4L2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66050-1-PBS. Detection antibody: 66050-2-PBS. Standard:Ag9233. Range: 0.098-100 ng/mL.

NDUFA4L2 Monoclonal antibody, PBS Only (Capture)

NDUFA4L2 Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 66050-1-PBS

产品说明书

CloneNo. 1G1H10

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

别名

NUOMS, NADH-ubiquinone oxidoreductase MLRQ subunit homolog, NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 4-like 2, 1G1H10

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy NDUFA4L2 Ready-To-Use IHC Kit. NDUFA4L2 primary antibody included.

产品信息

66050-1-PBS targets NDUFA4L2 as part of a matched antibody pair:

MP50529-1: 66050-1-PBS capture and 66050-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原 NDUFA4L2 fusion protein Ag9233 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 4-like 2
别名 NUOMS, NADH-ubiquinone oxidoreductase MLRQ subunit homolog, NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 4-like 2, 1G1H10
计算分子量 87 aa, 10 kDa
观测分子量 10 kDa
GenBank蛋白编号BC011910
基因名称 NDUFA4L2
Gene ID (NCBI) 56901
RRIDAB_11045656
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9NRX3
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NDUFA4L2, also named as NUOMS, is a HIF-1α target gene that localizes to the mitochondria. It downregulates oxygen consumption and Complex I activity in hypoxia. NDUFA4L2 is involved in hypoxic adaptation by decreasing mitochondrial ROS production.