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NDUFA4L2 Monoclonal antibody, PBS Only (Capture)

NDUFA4L2 Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1G1H10

Cat no : 66050-1-PBS

Print datasheet

Synonyms

NUOMS, NADH-ubiquinone oxidoreductase MLRQ subunit homolog, NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 4-like 2, 1G1H10

验证数据展示

  • IF Staining of OS-RC-2 using 66050-1-Ig (same clone as 66050-1-PBS)

    IF Staining of OS-RC-2 using 66050-1-Ig (same clone as 66050-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed OS-RC-2 cells using NDUFA4L2 antibody (66050-1-Ig, Clone: 1G1H10 ) at dilution of 1:1200 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 66050-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 66050-1-Ig (same clone as 66050-1-PBS)

    WB analysis of HeLa using 66050-1-Ig (same clone as 66050-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66050-1-Ig (NDUFA4L2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66050-1-PBS in a different storage buffer formulation.

  • IF Staining of HUVEC using 66050-1-Ig (same clone as 66050-1-PBS)

    IF Staining of HUVEC using 66050-1-Ig (same clone as 66050-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using NDUFA4L2 antibody (66050-1-Ig, Clone: 1G1H10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66050-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66050-1-Ig (same clone as 66050-1-PBS)

    IHC staining of human breast cancer using 66050-1-Ig (same clone as 66050-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66050-1-Ig (NDUFA4L2 Antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66050-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NDUFA4L2 antibody included
  • IHC staining of human breast cancer using 66050-1-Ig (same clone as 66050-1-PBS)

    IHC staining of human breast cancer using 66050-1-Ig (same clone as 66050-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66050-1-Ig (NDUFA4L2 Antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66050-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NDUFA4L2 antibody included
  • Cytometric bead array standard curve of MP50529-1

    Cytometric bead array standard curve of MP50529-1

    Cytometric bead array standard curve of MP50529-1, NDUFA4L2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66050-1-PBS. Detection antibody: 66050-2-PBS. Standard:Ag9233. Range: 0.098-100 ng/mL.

Planning an IHC experiment? We recommend our IHCeasy NDUFA4L2 Ready-To-Use IHC Kit. NDUFA4L2 primary antibody included.

产品信息

66050-1-PBS targets NDUFA4L2 as part of a matched antibody pair:

MP50529-1: 66050-1-PBS capture and 66050-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human
Immunogen NDUFA4L2 fusion protein Ag9233 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 4-like 2
Synonyms NUOMS, NADH-ubiquinone oxidoreductase MLRQ subunit homolog, NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 4-like 2, 1G1H10
Calculated Molecular Weight 87 aa, 10 kDa
Observed Molecular Weight 10 kDa
GenBank Accession NumberBC011910
Gene Symbol NDUFA4L2
Gene ID (NCBI) 56901
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9NRX3
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NDUFA4L2, also named as NUOMS, is a HIF-1α target gene that localizes to the mitochondria. It downregulates oxygen consumption and Complex I activity in hypoxia. NDUFA4L2 is involved in hypoxic adaptation by decreasing mitochondrial ROS production.