NDUFA4L2 Monoclonal antibody
NDUFA4L2 Monoclonal Antibody for IF/ICC, IHC, WB, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
human
Applications
IF/ICC, IHC, WB, ELISA
Conjugate
Unconjugated
CloneNo.
1G1H10
验证数据展示
经过测试的应用
| Positive WB detected in | Hela cells |
| Positive IHC detected in | human breast cancer tissue, human renal cell carcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HUVEC cells, HeLa cells, MCF-7 cells |
Planning an IHC experiment? We recommend our IHCeasy NDUFA4L2 Ready-To-Use IHC Kit. NDUFA4L2 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:1000 |
| Immunofluorescence (IF) | IF : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| IHC | See 1 publications below |
产品信息
The immunogen of 66050-1-Ig is NDUFA4L2 Fusion Protein expressed in E. coli.
| Tested Applications | IF/ICC, IHC, WB, ELISA Application Description |
| Cited Applications | WB, IHC |
| Tested Reactivity | human |
| Cited Reactivity | human |
| Immunogen | NDUFA4L2 fusion protein Ag9233 种属同源性预测 |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 4-like 2 |
| Synonyms | NDUFA4L2, NUOMS |
| Calculated Molecular Weight | 87 aa, 10 kDa |
| Observed Molecular Weight | 10 kDa |
| GenBank Accession Number | BC011910 |
| Gene Symbol | NDUFA4L2 |
| Gene ID (NCBI) | 56901 |
| RRID | AB_11045656 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9NRX3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NDUFA4L2, also named as NUOMS, is a HIF-1α target gene that localizes to the mitochondria. It downregulates oxygen consumption and Complex I activity in hypoxia. NDUFA4L2 is involved in hypoxic adaptation by decreasing mitochondrial ROS production.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NDUFA4L2 antibody 66050-1-Ig | Download protocol |
| IHC protocol for NDUFA4L2 antibody 66050-1-Ig | Download protocol |
| IF protocol for NDUFA4L2 antibody 66050-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Elife Molecular classification and tumor microenvironment characteristics in pheochromocytomas | ||
Cancer Biol Ther NDUFA4L2 reduces mitochondrial respiration resulting in defective lysosomal trafficking in clear cell renal cell carcinoma |
