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NDUFA4L2 Monoclonal antibody

NDUFA4L2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1G1H10

Cat no : 66050-1-Ig

Print datasheet

Synonyms

NUOMS, NADH-ubiquinone oxidoreductase MLRQ subunit homolog, NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 4-like 2, 1G1H10

验证数据展示

  • IF Staining of OS-RC-2 using 66050-1-Ig

    IF Staining of OS-RC-2 using 66050-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed OS-RC-2 cells using NDUFA4L2 antibody (66050-1-Ig, Clone: 1G1H10 ) at dilution of 1:1200 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

  • WB analysis of HeLa using 66050-1-Ig

    WB analysis of HeLa using 66050-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 66050-1-Ig (NDUFA4L2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HUVEC using 66050-1-Ig

    IF Staining of HUVEC using 66050-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using NDUFA4L2 antibody (66050-1-Ig, Clone: 1G1H10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IHC staining of human breast cancer using 66050-1-Ig

    IHC staining of human breast cancer using 66050-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66050-1-Ig (NDUFA4L2 Antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NDUFA4L2 antibody included
  • IHC staining of human breast cancer using 66050-1-Ig

    IHC staining of human breast cancer using 66050-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66050-1-Ig (NDUFA4L2 Antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NDUFA4L2 antibody included

经过测试的应用

Positive WB detected inHela cells
Positive IHC detected inhuman breast cancer tissue, human renal cell carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHUVEC cells, OS-RC-2 cells, MCF-7 cells, HeLa cells
Planning an IHC experiment? We recommend our IHCeasy NDUFA4L2 Ready-To-Use IHC Kit. NDUFA4L2 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66050-1-Ig targets NDUFA4L2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human
Cited Reactivityhuman
Immunogen NDUFA4L2 fusion protein Ag9233 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 4-like 2
Synonyms NUOMS, NADH-ubiquinone oxidoreductase MLRQ subunit homolog, NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 4-like 2, 1G1H10
Calculated Molecular Weight 87 aa, 10 kDa
Observed Molecular Weight 10 kDa
GenBank Accession NumberBC011910
Gene Symbol NDUFA4L2
Gene ID (NCBI) 56901
RRIDAB_11045656
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9NRX3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NDUFA4L2, also named as NUOMS, is a HIF-1α target gene that localizes to the mitochondria. It downregulates oxygen consumption and Complex I activity in hypoxia. NDUFA4L2 is involved in hypoxic adaptation by decreasing mitochondrial ROS production.

实验方案

Product Specific Protocols
WB protocol for NDUFA4L2 antibody 66050-1-IgDownload protocol
IHC protocol for NDUFA4L2 antibody 66050-1-IgDownload protocol
IF protocol for NDUFA4L2 antibody 66050-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Elife

Molecular classification and tumor microenvironment characteristics in pheochromocytomas

Authors - Sen Qin
View Article
humanWB

Cancer Biol Ther

NDUFA4L2 reduces mitochondrial respiration resulting in defective lysosomal trafficking in clear cell renal cell carcinoma

Authors - Jaclyn M Kubala
View Article