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CoraLite®594-conjugated NDUFA4L2 Monoclonal antibody

NDUFA4L2 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1G1H10

Cat no : CL594-66050

Print datasheet

Synonyms

FLJ26118, NDUFA4L2, NUOMS

验证数据展示

  • IF Staining of MCF-7 using CL594-66050

    IF Staining of MCF-7 using CL594-66050

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CoraLite®594-conjugated NDUFA4L2 antibody (CL594-66050, Clone: 1G1H10 ) at dilution of 1:100, 488phalloidin (green).

经过测试的应用

Positive IF/ICC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy NDUFA4L2 Ready-To-Use IHC Kit. NDUFA4L2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66050-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of CL594-66050 is NDUFA4L2 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC Application Description
Tested Reactivity human
Immunogen NDUFA4L2 fusion protein Ag9233 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 4-like 2
Synonyms FLJ26118, NDUFA4L2, NUOMS
Calculated Molecular Weight 87 aa, 10 kDa
Observed Molecular Weight 10 kDa
GenBank Accession NumberBC011910
Gene Symbol NDUFA4L2
Gene ID (NCBI) 56901
RRIDAB_2883492
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9NRX3
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NDUFA4L2, also named as NUOMS, is a HIF-1α target gene that localizes to the mitochondria. It downregulates oxygen consumption and Complex I activity in hypoxia. NDUFA4L2 is involved in hypoxic adaptation by decreasing mitochondrial ROS production.

实验方案

Product Specific Protocols
IF protocol for CL594 NDUFA4L2 antibody CL594-66050Download protocol
Standard Protocols
Click here to view our Standard Protocols