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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 68362-1-Ig (same clone as 68362-1-PBS)

    WB analysis using 68362-1-Ig (same clone as 68362-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68362-1-Ig (NDUFB7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68362-1-PBS in a different storage buffer formulation.

  • FC experiment of HEK-293 using 68362-1-Ig (same clone as 68362-1-PBS)

    FC experiment of HEK-293 using 68362-1-Ig (same clone as 68362-1-PBS)

    1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug Anti-Human NDUFB7 (68362-1-Ig, Clone:1H8C1) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68362-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse heart using 68362-1-Ig (same clone as 68362-1-PBS)

    IHC staining of mouse heart using 68362-1-Ig (same clone as 68362-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 68362-1-Ig (NDUFB7 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68362-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse heart using 68362-1-Ig (same clone as 68362-1-PBS)

    IHC staining of mouse heart using 68362-1-Ig (same clone as 68362-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 68362-1-Ig (NDUFB7 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68362-1-PBS in a different storage buffer formulation.

NDUFB7 Monoclonal antibody, PBS Only

NDUFB7 Monoclonal Antibody for Indirect ELISA, IHC, WB
Cat No. 68362-1-PBS

产品说明书

CloneNo. 1H8C1

宿主/亚型

Mouse / IgG1

种属反应性

rabbit, pig, rat, mouse, human

应用

Indirect ELISA, IHC, WB

别名

Complex I B18, CI-B18, CI B18, Cell adhesion protein SQM1, B18

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

68362-1-PBS targets NDUFB7 in Indirect ELISA, IHC, WB applications and shows reactivity with rabbit, pig, rat, mouse, human samples.

经测试应用 Indirect ELISA, IHC, WB Application Description
经测试反应性 rabbit, pig, rat, mouse, human
免疫原 NDUFB7 fusion protein Ag6720 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 7, 18kDa
别名 Complex I B18, CI-B18, CI B18, Cell adhesion protein SQM1, B18
计算分子量 16 kDa
观测分子量18 kDa
GenBank蛋白编号BC002595
基因名称 NDUFB7
Gene ID (NCBI) 4713
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP17568
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NDUFB7(NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7) is also named as CI-B18 and belongs to the complex I NDUFB7 subunit family. It is an accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), which couples the oxidation of NADH to the reduction of ubiquinone and the translocation of protons across the inner membrane. NDUFB7 protein lacks a mitochondrial targeting signal and transmembrane domains. However, it has a Cx(9)C motif that includes an intermembrane space targeting signal.(PMID:21310150).