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NDUFB7 Monoclonal antibody

NDUFB7 Monoclonal Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IHC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1H8C1

Cat no : 68362-1-Ig

Print datasheet

Synonyms

Complex I B18, CI-B18, CI B18, Cell adhesion protein SQM1, B18

验证数据展示

  • WB analysis using 68362-1-Ig

    WB analysis using 68362-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68362-1-Ig (NDUFB7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of HEK-293 using 68362-1-Ig

    FC experiment of HEK-293 using 68362-1-Ig

    1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug Anti-Human NDUFB7 (68362-1-Ig, Clone:1H8C1) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IHC staining of mouse heart using 68362-1-Ig

    IHC staining of mouse heart using 68362-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 68362-1-Ig (NDUFB7 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse heart using 68362-1-Ig

    IHC staining of mouse heart using 68362-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 68362-1-Ig (NDUFB7 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inLNCaP cells, MCF-7 cells, HeLa cells, HEK-293 cells, PC-3 cell, pig heart tissue, rabbit heart tissue, rat heart tissue, mouse heart tissue
Positive IHC detected inmouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inHEK-293 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68362-1-Ig targets NDUFB7 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Applications WB, IHC, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse, rat, pig, rabbit
Immunogen NDUFB7 fusion protein Ag6720 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 7, 18kDa
Synonyms Complex I B18, CI-B18, CI B18, Cell adhesion protein SQM1, B18
Calculated Molecular Weight 16 kDa
Observed Molecular Weight18 kDa
GenBank Accession NumberBC002595
Gene Symbol NDUFB7
Gene ID (NCBI) 4713
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP17568
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NDUFB7(NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7) is also named as CI-B18 and belongs to the complex I NDUFB7 subunit family. It is an accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), which couples the oxidation of NADH to the reduction of ubiquinone and the translocation of protons across the inner membrane. NDUFB7 protein lacks a mitochondrial targeting signal and transmembrane domains. However, it has a Cx(9)C motif that includes an intermembrane space targeting signal.(PMID:21310150).

实验方案

Product Specific Protocols
WB protocol for NDUFB7 antibody 68362-1-IgDownload protocol
IHC protocol for NDUFB7 antibody 68362-1-IgDownload protocol
FC protocol for NDUFB7 antibody 68362-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols