验证数据展示
经过测试的应用
| Positive FC (Intra) detected in | HEK-293 cells |
For other applications, we recommend the unconjugated version of this antibody, 68362-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-68362 targets NDUFB7 in FC (Intra) applications and shows reactivity with human, mouse, rat, pig, rabbit samples.
| 经测试应用 | FC (Intra) Application Description |
| 经测试反应性 | human, mouse, rat, pig, rabbit |
| 免疫原 | NDUFB7 fusion protein Ag6720 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 7, 18kDa |
| 别名 | Complex I B18, CI-B18, CI B18, Cell adhesion protein SQM1, B18 |
| 计算分子量 | 16 kDa |
| 观测分子量 | 18 kDa |
| GenBank蛋白编号 | BC002595 |
| 基因名称 | NDUFB7 |
| Gene ID (NCBI) | 4713 |
| RRID | AB_3084479 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P17568 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NDUFB7(NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7) is also named as CI-B18 and belongs to the complex I NDUFB7 subunit family. It is an accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), which couples the oxidation of NADH to the reduction of ubiquinone and the translocation of protons across the inner membrane. NDUFB7 protein lacks a mitochondrial targeting signal and transmembrane domains. However, it has a Cx(9)C motif that includes an intermembrane space targeting signal.(PMID:21310150).
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 488 NDUFB7 antibody CL488-68362 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |