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CoraLite® Plus 488-conjugated NDUFB7 Monoclonal antibody

NDUFB7 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1H8C1

Cat no : CL488-68362

Print datasheet

Synonyms

Complex I B18, CI-B18, CI B18, Cell adhesion protein SQM1, B18

验证数据展示

  • FC experiment of HEK-293 using CL488-68362

    FC experiment of HEK-293 using CL488-68362

    1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human NDUFB7 (CL488-68362, Clone:1H8C1) (red), or 0.4 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (MOPC-21) (CL488-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC (Intra) detected inHEK-293 cells
For other applications, we recommend the unconjugated version of this antibody, 68362-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68362 targets NDUFB7 in FC (Intra) applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human, mouse, rat, pig, rabbit
Immunogen NDUFB7 fusion protein Ag6720 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 7, 18kDa
Synonyms Complex I B18, CI-B18, CI B18, Cell adhesion protein SQM1, B18
Calculated Molecular Weight 16 kDa
Observed Molecular Weight18 kDa
GenBank Accession NumberBC002595
Gene Symbol NDUFB7
Gene ID (NCBI) 4713
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP17568
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NDUFB7(NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7) is also named as CI-B18 and belongs to the complex I NDUFB7 subunit family. It is an accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), which couples the oxidation of NADH to the reduction of ubiquinone and the translocation of protons across the inner membrane. NDUFB7 protein lacks a mitochondrial targeting signal and transmembrane domains. However, it has a Cx(9)C motif that includes an intermembrane space targeting signal.(PMID:21310150).

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 NDUFB7 antibody CL488-68362Download protocol
Standard Protocols
Click here to view our Standard Protocols