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验证数据展示

  • WB analysis of HepG2 using 66053-1-Ig

    WB analysis of HepG2 using 66053-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66053-1-Ig (NDUFS5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 66053-1-Ig

    IHC staining of human kidney using 66053-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney using 66053-1-Ig(NDUFS5 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human kidney using 66053-1-Ig

    IHC staining of human kidney using 66053-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney using 66053-1-Ig(NDUFS5 antibody) at dilution of 1:50 (under 40x lens).

NDUFS5 Monoclonal antibody

NDUFS5 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 66053-1-Ig

产品说明书

CloneNo. 2C2A5

宿主/亚型

Mouse / IgG2b

种属反应性

human

应用

WB, IHC, ELISA

别名

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, L02 cells, HEK-293 cells, fetal human brain tissue
Positive IHC detected inhuman kidney tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66053-1-Ig targets NDUFS5 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 NDUFS5 fusion protein Ag7663 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 NADH dehydrogenase (ubiquinone) Fe-S protein 5, 15kDa (NADH-coenzyme Q reductase)
别名
计算分子量 13 kDa
观测分子量 15 kDa
GenBank蛋白编号BC001884
基因名称 NDUFS5
Gene ID (NCBI) 4725
RRIDAB_11043342
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO43920
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NDUFS5 gene is a member of the NADH dehydrogenase (ubiquinone) iron-sulfur protein family. The encoded protein is a subunit of the NADH:ubiquinone oxidoreductase (complex I), the first enzyme complex in the electron transport chain located in the inner mitochondrial membrane. The NDUFS5 mRNA is expressed ubiquitously in human tissues, with a relative higher expression in human heart, skeletal muscle, liver, kidney and fetal heart (PMID: 10070614). The NDUFS5 (15 kDa) subunit located at mitochondrion and contains two C-X9-C motifs that are predicted to form a helix-coil-helix structure, permitting the formation of intramolecular disulfide bonds (PMID: 21310150). Proteinteach’s NDUFS5 antibody 66053-1-Ig is capable of detecting the 15 kDa protein in Western blotting.

实验方案

Product Specific Protocols
WB protocol for NDUFS5 antibody 66053-1-IgDownload protocol
IHC protocol for NDUFS5 antibody 66053-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols