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NDUFS5 Monoclonal antibody, PBS Only (Detector)

NDUFS5 Monoclonal Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2C2A5

Cat no : 66053-1-PBS

Print datasheet

Synonyms

NADH dehydrogenase [ubiquinone] iron-sulfur protein 5, Complex I-15 kDa, Complex I 15 kDa, CI-15 kDa, CI 15 kDa

验证数据展示

  • WB analysis of HepG2 using 66053-1-Ig (same clone as 66053-1-PBS)

    WB analysis of HepG2 using 66053-1-Ig (same clone as 66053-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66053-1-Ig (NDUFS5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66053-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 66053-1-Ig (same clone as 66053-1-PBS)

    IHC staining of human kidney using 66053-1-Ig (same clone as 66053-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 66053-1-Ig(NDUFS5 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66053-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 66053-1-Ig (same clone as 66053-1-PBS)

    IHC staining of human kidney using 66053-1-Ig (same clone as 66053-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 66053-1-Ig(NDUFS5 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66053-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50607-1

    Cytometric bead array standard curve of MP50607-1

    Cytometric bead array standard curve of MP50607-1, NDUFS5 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66053-2-PBS. Detection antibody: 66053-1-PBS. Standard:Ag7663. Range: 3.125-100 ng/mL.

产品信息

66053-1-PBS targets NDUFS5 as part of a matched antibody pair:

MP50607-1: 66053-2-PBS capture and 66053-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IHC, Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human
Immunogen NDUFS5 fusion protein Ag7663 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name NADH dehydrogenase (ubiquinone) Fe-S protein 5, 15kDa (NADH-coenzyme Q reductase)
Synonyms NADH dehydrogenase [ubiquinone] iron-sulfur protein 5, Complex I-15 kDa, Complex I 15 kDa, CI-15 kDa, CI 15 kDa
Calculated Molecular Weight 13 kDa
Observed Molecular Weight 15 kDa
GenBank Accession NumberBC001884
Gene Symbol NDUFS5
Gene ID (NCBI) 4725
RRIDAB_11043342
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO43920
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NDUFS5 gene is a member of the NADH dehydrogenase (ubiquinone) iron-sulfur protein family. The encoded protein is a subunit of the NADH:ubiquinone oxidoreductase (complex I), the first enzyme complex in the electron transport chain located in the inner mitochondrial membrane. The NDUFS5 mRNA is expressed ubiquitously in human tissues, with a relative higher expression in human heart, skeletal muscle, liver, kidney and fetal heart (PMID: 10070614). The NDUFS5 (15 kDa) subunit located at mitochondrion and contains two C-X9-C motifs that are predicted to form a helix-coil-helix structure, permitting the formation of intramolecular disulfide bonds (PMID: 21310150). Proteinteach's NDUFS5 antibody 66053-1-Ig is capable of detecting the 15 kDa protein in Western blotting.