验证数据展示
产品信息
83112-1-PBS targets NEDD4 in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IF/ICC, IP, Indirect ELISA Application Description |
Tested Reactivity | human, mouse, rat |
Immunogen | NEDD4 fusion protein Ag16291 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | neural precursor cell expressed, developmentally down-regulated 4 |
Synonyms | KIAA0093, HECT-type E3 ubiquitin transferase NEDD4, EC:2.3.2.26, E3 ubiquitin-protein ligase NEDD4, Cell proliferation-inducing gene 53 protein |
Calculated Molecular Weight | 1319 aa, 149 kDa |
Observed Molecular Weight | 115 kDa |
GenBank Accession Number | BC152452 |
Gene Symbol | NEDD4 |
Gene ID (NCBI) | 4734 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P46934 |
Storage Buffer | PBS Only |
Storage Conditions | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
背景介绍
NEDD4 and similar proteins discovered subsequently became a family of HECT ligases, comprising 9 human proteins including NEDD4, NEDD4-2 (NEDD4L), ITCH, SMURF1, SMURF2, WWP1, WWP2, NEDL1 and NEDL2. NEDD4 is a highly evolutionarily conserved protein from yeast to man, and was initially cloned as a highly expressed gene in the early embryonic brain. NEDD4 is frequently overexpressed in many different types of cancer, and decreased levels of NEDD4 can also be associated with cancer. It can be a potential therapeutic target for the treatment of human cancer.(PMID: 25527121). The human NEDD4 gene is located on chromosome 15q21.3 and comprises 30 exons (HGNC:7727) shown to encode a ~120 KDa protein. Otherwise there is a 75 kDa isoform in addition to full length NEDD4 (PMID: 24907641).