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验证数据展示

  • IF Staining of Jurkat using CL488-67276

    IF Staining of Jurkat using CL488-67276

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CoraLite® Plus 488 NEDD4L antibody (CL488-67276, Clone: 2F6H6 ) at dilution of 1:200.

  • FC experiment of Jurkat using CL488-67276

    FC experiment of Jurkat using CL488-67276

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human NEDD4L (CL488-67276, Clone:2F6H6) (red), or 0.4 ug x. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated NEDD4L Monoclonal antibody

NEDD4L Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-67276

产品说明书

CloneNo. 2F6H6

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

IF/ICC, FC (Intra)

别名

KIAA0439, hNEDD4-2, HECT-type E3 ubiquitin transferase NED4L, EC:2.3.2.26, E3 ubiquitin-protein ligase NEDD4-like

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inJurkat cells
Positive FC (Intra) detected inJurkat cells
For other applications, we recommend the unconjugated version of this antibody, 67276-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67276 targets NEDD4L in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 NEDD4L fusion protein Ag4565 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 neural precursor cell expressed, developmentally down-regulated 4-like
别名 KIAA0439, hNEDD4-2, HECT-type E3 ubiquitin transferase NED4L, EC:2.3.2.26, E3 ubiquitin-protein ligase NEDD4-like
计算分子量 854 aa, 96 kDa
观测分子量 112 kDa, 125 kDa
GenBank蛋白编号BC032597
基因名称 NEDD4L
Gene ID (NCBI) 23327
RRIDAB_3084333
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ96PU5
储存缓冲液 PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NEDD4L(E3 ubiquitin-protein ligase NEDD4-like) is also named as KIAA0439, NEDL3, Nedd4-2. It accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substratesand and is the principal ubiquitin ligase that selectively targets activated SMAD2 and SMAD3 for destruction (PMID:19917253). Nedd4L binds the PY motif of ENaC COOH terminals and catalyzes ubiquitination of the NH2 terminus of the protein for subsequent degradation (PMID:18268134). It has some isoforms produced by alternative splicing. This antibody may have cross reaction with NEDD4 due to the high homology of the antigen.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 NEDD4L antibody CL488-67276Download protocol
FC protocol for CL Plus 488 NEDD4L antibody CL488-67276Download protocol
Standard Protocols
Click here to view our Standard Protocols