NF-H/NF200 Polyclonal antibody
NF-H/NF200 Polyclonal Antibody for WB, IHC, IF-P, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IHC, IF-P, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | mouse brain tissue, rat brain tissue |
Positive IHC detected in | rat brain tissue, human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | rat brain tissue |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:3000 |
Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
18934-1-AP targets NF-H/NF200 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IHC, IF-P, ELISA Application Description |
Cited Applications | WB, IHC, IF |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, canine |
Immunogen | NF-H/NF200 fusion protein Ag13517 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | neurofilament, heavy polypeptide |
Synonyms | 200 kDa neurofilament protein, NEFH, NEH, Neurofilament H, NF, NF H, NF200, NF-H |
Calculated Molecular Weight | 112 kDa |
Observed Molecular Weight | 200 kDa, 140-160 kDa |
GenBank Accession Number | BC014185 |
Gene Symbol | NF-H |
Gene ID (NCBI) | 4744 |
RRID | AB_10640801 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P12036 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for NF-H/NF200 antibody 18934-1-AP | Download protocol |
IHC protocol for NF-H/NF200 antibody 18934-1-AP | Download protocol |
IF protocol for NF-H/NF200 antibody 18934-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
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