NF-H/NF200 Polyclonal antibody

NF-H/NF200 Polyclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 18934-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (1)

应用

WB, IHC, IF-P, ELISA and More (1)

NEFH, Neurofilament heavy polypeptide, NF200, NF-H, neurofilament heavy chain

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inrat brain tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inrat brain tissue, mouse brain tissue
Planning an IHC experiment? We recommend our IHCeasy NF-H/NF200 Ready-To-Use IHC Kit. NF-H/NF200 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18934-1-AP targets NF-H/NF200 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat, canine
免疫原 NF-H/NF200 fusion protein Ag13517 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 neurofilament, heavy polypeptide
别名 NEFH, Neurofilament heavy polypeptide, NF200, NF-H, neurofilament heavy chain
计算分子量 112 kDa
观测分子量 200 kDa, 140-160 kDa
GenBank蛋白编号BC014185
基因名称 NF-H
Gene ID (NCBI) 4744
RRIDAB_10640801
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP12036
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NEFH (NF200), also named as KIAA0845 and NFH, belongs to the intermediate filament family. It has an important function in mature axons that is not subserved by the two smaller NF proteins. Neurofilaments are the 10 nm intermediate filaments found specifically in neurons. They are a major component of the cell's cytoskeleton, and provide support for normal axonal radial growth. Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. The names given to the three major neurofilament subunits are based upon the apparent molecular weight of the mammalian subunits on SDS-PAGE: NF-L, 65-68 kDa; NF-M,145-160 kDa and NF-H, 200-220 kDa. This antibody can recognize both NEFH and NEFM.

实验方案

Product Specific Protocols
WB protocol for NF-H/NF200 antibody 18934-1-APDownload protocol
IHC protocol for NF-H/NF200 antibody 18934-1-APDownload protocol
IF protocol for NF-H/NF200 antibody 18934-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIHC

Cell Metab

Gut microbiota modulate distal symmetric polyneuropathy in patients with diabetes

Authors - Junpeng Yang
humanIHC

Small Methods

Polydopamine-Decorated PLCL Conduit to Induce Synergetic Effect of Electrical Stimulation and Topological Morphology for Peripheral Nerve Regeneration

Authors - Shunyi Lu
ratIHC

Biofabrication

Pulsed electromagnetic field-assisted reduced graphene oxide composite 3D printed nerve scaffold promotes sciatic nerve regeneration in rats

Authors - Zichao Wang
ratIF

Cell Rep

TMC7 functions as a suppressor of Piezo2 in primary sensory neurons blunting peripheral mechanotransduction

Authors - Xiaoxue Zhang

Acta Neuropathol Commun

A robust TDP-43 knock-in mouse model of ALS.

Authors - Shih-Ling Huang
mouseWB

PLoS Genet

Tracking the Fragile X Mental Retardation Protein in a Highly Ordered Neuronal RiboNucleoParticles Population: A Link between Stalled Polyribosomes and RNA Granules.

Authors - Rachid El Fatimy
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