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NF-M Monoclonal antibody

NF-M Monoclonal Antibody for WB, IHC, ELISA, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA, FC (Intra)

Conjugate

Unconjugated

CloneNo.

2E3B12

Cat no : 66396-1-Ig

Print datasheet

Synonyms

NEFM, NF M, neurofilament, NEF3, 2E3B12

验证数据展示

  • IHC staining of mouse brain using 66396-1-Ig

    IHC staining of mouse brain using 66396-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66396-1-Ig (NF-M antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NF-M antibody included
  • IHC staining of mouse brain using 66396-1-Ig

    IHC staining of mouse brain using 66396-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66396-1-Ig (NF-M antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NF-M antibody included
  • IHC staining of mouse cerebellum using 66396-1-Ig

    IHC staining of mouse cerebellum using 66396-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 66396-1-Ig (NF-M antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NF-M antibody included
  • WB analysis of rat brain using 66396-1-Ig

    WB analysis of rat brain using 66396-1-Ig

    Lysates of rat brain, mouse brain tissues and PC-12 cells were subjected to SDS PAGE followed by western blot with 66396-1-Ig (NEFM Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • FC experiment of PC-12 using 66396-1-Ig

    FC experiment of PC-12 using 66396-1-Ig

    1X10^6 PC-12 cells were intracellularly stained with 0.4 ug Anti-Human NF-M (66396-1-Ig, Clone:2E3B12) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inrat brain tissue, rat brain, mouse brain tissue, PC-12 cells
Positive IHC detected inmouse brain tissue, mouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inPC-12 cells
Planning an IHC experiment? We recommend our IHCeasy NF-M Ready-To-Use IHC Kit. NF-M primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:20000
Immunohistochemistry (IHC)IHC : 1:200-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66396-1-Ig targets NF-M in WB, IHC, ELISA, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen NF-M fusion protein Ag22709 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name neurofilament, medium polypeptide
Synonyms NEFM, NF M, neurofilament, NEF3, 2E3B12
Calculated Molecular Weight 102 kDa
Observed Molecular Weight 140 kDa
GenBank Accession NumberBC002421
Gene Symbol NF-M
Gene ID (NCBI) 4741
RRIDAB_2881770
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP07197
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NEFM, also named as NEF3 and NFM, belongs to the intermediate filament family. Neurofilaments are the 10 nm intermediate filaments found specifically in neurons. They are a major component of the cell's cytoskeleton, and provide support for normal axonal radial growth. Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. The names given to the three major neurofilament subunits are based upon the apparent molecular weight of the mammalian subunits on SDS-PAGE: NF-L, 65-68 kDa; NF-M,140-160 kDa and NF-H, 200-220 kDa.

实验方案

Product Specific Protocols
WB protocol for NF-M antibody 66396-1-IgDownload protocol
IHC protocol for NF-M antibody 66396-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols