验证数据展示
经过测试的应用
| Positive FC (Intra) detected in | PC-12 cells |
| Positive FC detected in | PC-12 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
| Flow Cytometry (FC) | FC : 0.80 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-66396 targets NF-M in FC (Intra) applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | FC (Intra) Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | NF-M fusion protein Ag22709 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | neurofilament, medium polypeptide |
| 别名 | 160 kDa neurofilament protein, NEF3, NEFM, neurofilament, Neurofilament 3, NF, NF M, NFM, NF-M |
| 计算分子量 | 102 kDa |
| 观测分子量 | 140 kDa |
| GenBank蛋白编号 | BC002421 |
| 基因名称 | NF-M |
| Gene ID (NCBI) | 4741 |
| RRID | AB_3672911 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P07197 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NEFM, also named as NEF3 and NFM, belongs to the intermediate filament family. Neurofilaments are the 10 nm intermediate filaments found specifically in neurons. They are a major component of the cell's cytoskeleton, and provide support for normal axonal radial growth. Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. The names given to the three major neurofilament subunits are based upon the apparent molecular weight of the mammalian subunits on SDS-PAGE: NF-L, 65-68 kDa; NF-M,140-160 kDa and NF-H, 200-220 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 488 NF-M antibody CL488-66396 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |