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CoraLite® Plus 488-conjugated NF-M Monoclonal antibody

NF-M Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2E3B12

Cat no : CL488-66396

Print datasheet

Synonyms

160 kDa neurofilament protein, NEF3, NEFM, neurofilament, Neurofilament 3, NF, NF M, NFM, NF-M

验证数据展示

  • FC experiment of PC-12 using CL488-66396

    FC experiment of PC-12 using CL488-66396

    1x10^6 PC-12 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human NF-M (CL488-66396, Clone:2E3B12)(red), or 0.8 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (1F8D3) (CL488-66360-1, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC (Intra) detected inPC-12 cells
Positive FC detected inPC-12 cells
Planning an IHC experiment? We recommend our IHCeasy NF-M Ready-To-Use IHC Kit. NF-M primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66396-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66396 targets NF-M in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen NF-M fusion protein Ag22709 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name neurofilament, medium polypeptide
Synonyms 160 kDa neurofilament protein, NEF3, NEFM, neurofilament, Neurofilament 3, NF, NF M, NFM, NF-M
Calculated Molecular Weight 102 kDa
Observed Molecular Weight 140 kDa
GenBank Accession NumberBC002421
Gene Symbol NF-M
Gene ID (NCBI) 4741
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP07197
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NEFM, also named as NEF3 and NFM, belongs to the intermediate filament family. Neurofilaments are the 10 nm intermediate filaments found specifically in neurons. They are a major component of the cell's cytoskeleton, and provide support for normal axonal radial growth. Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. The names given to the three major neurofilament subunits are based upon the apparent molecular weight of the mammalian subunits on SDS-PAGE: NF-L, 65-68 kDa; NF-M,140-160 kDa and NF-H, 200-220 kDa.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 NF-M antibody CL488-66396Download protocol
Standard Protocols
Click here to view our Standard Protocols