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验证数据展示

  • WB analysis of HeLa using 67012-1-Ig

    WB analysis of HeLa using 67012-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 67012-1-Ig (NEIL1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse spleen using 67012-1-Ig

    IHC staining of mouse spleen using 67012-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 67012-1-Ig (NEIL1 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 67012-1-Ig

    IF Staining of HepG2 using 67012-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 67012-1-Ig (NEIL1 antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L).

NEIL1 Monoclonal antibody

NEIL1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67012-1-Ig

产品说明书

CloneNo. 1C6D6

宿主/亚型

Mouse / IgG2a

种属反应性

Human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

DNA glycosylase/AP lyase Neil1, Endonuclease 8 like 1, Endonuclease VIII like 1, FLJ22402, FPG1, hFPG1, NEH1, Nei homolog 1, Nei like protein 1, NEI1, NEIL1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, A375 cells, COLO 320 cells
Positive IHC detected inmouse spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:150-1:600
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67012-1-Ig targets NEIL1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 Human, mouse
免疫原 NEIL1 fusion protein Ag8307 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 nei endonuclease VIII-like 1 (E. coli)
别名 DNA glycosylase/AP lyase Neil1, Endonuclease 8 like 1, Endonuclease VIII like 1, FLJ22402, FPG1, hFPG1, NEH1, Nei homolog 1, Nei like protein 1, NEI1, NEIL1
计算分子量 390 aa, 44 kDa
观测分子量 44 kDa
GenBank蛋白编号BC010876
基因名称 NEIL1
Gene ID (NCBI) 79661
RRIDAB_2882329
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ96FI4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NEIL1, also named as NEH1 and FPG1, belongs to the FPG family. It is involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. NEIL1 acts as DNA glycosylase that recognizes and removes damaged bases. It has a preference for oxidized pyrimidines, such as thymine glycol, formamidopyrimidine (Fapy) and 5-hydroxyuracil. NEIL1 has marginal activity towards 8-oxoguanine. It has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. It cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates. NEIL1 has DNA glycosylase/lyase activity towards mismatched uracil and thymine, in particular in U:C and T:C mismatches. The increased BER activity of NEILs may represent an adaptive response against ROS-induced DNA damage resulting from aniline exposure, and could be an important mechanism for the removal of oxidative DNA lesions. (PMID:21145906)

实验方案

Product Specific Protocols
WB protocol for NEIL1 antibody 67012-1-IgDownload protocol
IHC protocol for NEIL1 antibody 67012-1-IgDownload protocol
IF protocol for NEIL1 antibody 67012-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols