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Host
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Species Reactivity
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Species Reactivity
0
Conjugate
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Conjugate
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验证数据展示

  • WB analysis of HeLa using 67012-1-Ig (same clone as 67012-1-PBS)

    WB analysis of HeLa using 67012-1-Ig (same clone as 67012-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 67012-1-Ig (NEIL1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67012-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse spleen using 67012-1-Ig (same clone as 67012-1-PBS)

    IHC staining of mouse spleen using 67012-1-Ig (same clone as 67012-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 67012-1-Ig (NEIL1 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67012-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 67012-1-Ig (same clone as 67012-1-PBS)

    IF Staining of HepG2 using 67012-1-Ig (same clone as 67012-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 67012-1-Ig (NEIL1 antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67012-1-PBS in a different storage buffer formulation.

NEIL1 Monoclonal antibody, PBS Only

NEIL1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67012-1-PBS

产品说明书

CloneNo. 1C6D6

宿主/亚型

Mouse / IgG2a

种属反应性

Human, mouse

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

DNA glycosylase/AP lyase Neil1, Endonuclease 8 like 1, Endonuclease VIII like 1, FLJ22402, FPG1, hFPG1, NEH1, Nei homolog 1, Nei like protein 1, NEI1, NEIL1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

67012-1-PBS targets NEIL1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 Human, mouse
免疫原 NEIL1 fusion protein Ag8307 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 nei endonuclease VIII-like 1 (E. coli)
别名 DNA glycosylase/AP lyase Neil1, Endonuclease 8 like 1, Endonuclease VIII like 1, FLJ22402, FPG1, hFPG1, NEH1, Nei homolog 1, Nei like protein 1, NEI1, NEIL1
计算分子量 390 aa, 44 kDa
观测分子量 44 kDa
GenBank蛋白编号BC010876
基因名称 NEIL1
Gene ID (NCBI) 79661
RRIDAB_2882329
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ96FI4
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NEIL1, also named as NEH1 and FPG1, belongs to the FPG family. It is involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. NEIL1 acts as DNA glycosylase that recognizes and removes damaged bases. It has a preference for oxidized pyrimidines, such as thymine glycol, formamidopyrimidine (Fapy) and 5-hydroxyuracil. NEIL1 has marginal activity towards 8-oxoguanine. It has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. It cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates. NEIL1 has DNA glycosylase/lyase activity towards mismatched uracil and thymine, in particular in U:C and T:C mismatches. The increased BER activity of NEILs may represent an adaptive response against ROS-induced DNA damage resulting from aniline exposure, and could be an important mechanism for the removal of oxidative DNA lesions. (PMID:21145906)