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NEIL1 Monoclonal antibody, PBS Only

NEIL1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1C6D6

Cat no : 67012-1-PBS

Print datasheet

Synonyms

DNA glycosylase/AP lyase Neil1, Endonuclease 8 like 1, Endonuclease VIII like 1, FLJ22402, FPG1, hFPG1, NEH1, Nei homolog 1, Nei like protein 1, NEI1, NEIL1

验证数据展示

  • WB analysis of HeLa using 67012-1-Ig (same clone as 67012-1-PBS)

    WB analysis of HeLa using 67012-1-Ig (same clone as 67012-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 67012-1-Ig (NEIL1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67012-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse spleen using 67012-1-Ig (same clone as 67012-1-PBS)

    IHC staining of mouse spleen using 67012-1-Ig (same clone as 67012-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 67012-1-Ig (NEIL1 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67012-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 67012-1-Ig (same clone as 67012-1-PBS)

    IF Staining of HepG2 using 67012-1-Ig (same clone as 67012-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 67012-1-Ig (NEIL1 antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67012-1-PBS in a different storage buffer formulation.

产品信息

67012-1-PBS targets NEIL1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity Human, mouse
Immunogen NEIL1 fusion protein Ag8307 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name nei endonuclease VIII-like 1 (E. coli)
Synonyms DNA glycosylase/AP lyase Neil1, Endonuclease 8 like 1, Endonuclease VIII like 1, FLJ22402, FPG1, hFPG1, NEH1, Nei homolog 1, Nei like protein 1, NEI1, NEIL1
Calculated Molecular Weight 390 aa, 44 kDa
Observed Molecular Weight 44 kDa
GenBank Accession NumberBC010876
Gene Symbol NEIL1
Gene ID (NCBI) 79661
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ96FI4
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NEIL1, also named as NEH1 and FPG1, belongs to the FPG family. It is involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. NEIL1 acts as DNA glycosylase that recognizes and removes damaged bases. It has a preference for oxidized pyrimidines, such as thymine glycol, formamidopyrimidine (Fapy) and 5-hydroxyuracil. NEIL1 has marginal activity towards 8-oxoguanine. It has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. It cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates. NEIL1 has DNA glycosylase/lyase activity towards mismatched uracil and thymine, in particular in U:C and T:C mismatches. The increased BER activity of NEILs may represent an adaptive response against ROS-induced DNA damage resulting from aniline exposure, and could be an important mechanism for the removal of oxidative DNA lesions. (PMID:21145906)