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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 83376-7-RR (same clone as 83376-7-PBS)

    WB analysis using 83376-7-RR (same clone as 83376-7-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83376-7-RR (NEIL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83376-7-PBS in a different storage buffer formulation.

  • FC experiment of HEK-293 using 83376-7-RR (same clone as 83376-7-PBS)

    FC experiment of HEK-293 using 83376-7-RR (same clone as 83376-7-PBS)

    1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug NEIL1 Recombinant antibody (83376-7-RR, Clone:240322G10) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83376-7-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83376-7-RR

    Affinity and Kinetic Characterization of 83376-7-RR

    Biolayer interferometry (BLl) kinetic assays of 83376-7-RR against Human NEIL1 were performed. The affinity constant is 0.521 nM.

NEIL1 Recombinant antibody, PBS Only

NEIL1 Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 83376-7-PBS

产品说明书

CloneNo. 240322G10

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

NEI L1, Endonuclease VIII-like 1, Endonuclease 8-like 1, EC:3.2.2.-, DNA-(apurinic or apyrimidinic site) lyase Neil1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

83376-7-PBS targets NEIL1 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 NEIL1 fusion protein Ag2793 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 nei endonuclease VIII-like 1 (E. coli)
别名 NEI L1, Endonuclease VIII-like 1, Endonuclease 8-like 1, EC:3.2.2.-, DNA-(apurinic or apyrimidinic site) lyase Neil1
计算分子量 390 aa, 44 kDa
观测分子量40-45 kDa
GenBank蛋白编号BC010876
基因名称 NEIL1
Gene ID (NCBI) 79661
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ96FI4
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NEIL1, also named as NEH1 and FPG1, belongs to the FPG family. It is involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. NEIL1 acts as DNA glycosylase that recognizes and removes damaged bases. It has a preference for oxidized pyrimidines, such as thymine glycol, formamidopyrimidine (Fapy) and 5-hydroxyuracil. NEIL1 has marginal activity towards 8-oxoguanine. It has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. It cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates. NEIL1 has DNA glycosylase/lyase activity towards mismatched uracil and thymine, in particular in U:C and T:C mismatches. The increased BER activity of NEILs may represent an adaptive response against ROS-induced DNA damage resulting from aniline exposure, and could be an important mechanism for the removal of oxidative DNA lesions. (PMID:21145906)