验证数据展示
经过测试的应用
| Positive WB detected in | K-562 cells |
| Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy NEK2 Ready-To-Use IHC Kit. NEK2 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 5 publications below |
| WB | See 8 publications below |
| IHC | See 6 publications below |
产品信息
24171-1-AP targets NEK2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 | NEK2 fusion protein Ag21476 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | NIMA (never in mitosis gene a)-related kinase 2 |
| 别名 | NimA like protein kinase 1, Never in mitosis A-related kinase 2, NEK2A, HsPK21, HSPK 21 |
| 计算分子量 | 52 kDa |
| 观测分子量 | 48 kDa |
| GenBank蛋白编号 | BC043502 |
| 基因名称 | NEK2 |
| Gene ID (NCBI) | 4751 |
| RRID | AB_2687577 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P51955 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NEK2 antibody 24171-1-AP | Download protocol |
| IHC protocol for NEK2 antibody 24171-1-AP | Download protocol |
| IF protocol for NEK2 antibody 24171-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun NEK2 inhibition triggers anti-pancreatic cancer immunity by targeting PD-L1.
| ||
Curr Biol RPL10L Is Required for Male Meiotic Division by Compensating for RPL10 during Meiotic Sex Chromosome Inactivation in Mice. | ||
Biochim Biophys Acta Mol Cell Res NEK2 overexpression aggravates IL-22-induced keratinocyte proliferation and cytokine level increases and IMQ-induced psoriasis-like dermatitis
| ||
Int J Biol Sci NEK2 plays an active role in Tumorigenesis and Tumor Microenvironment in Non-Small Cell Lung Cancer.
| ||
Mol Carcinog NEK2 Promotes ESCC Malignant Progression by Inhibiting Cellular Senescence via the FOXM1/c-Myc/p27 Signaling Pathway
| ||
