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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated NEK2 Polyclonal antibody

NEK2 Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-24171

Print datasheet

Synonyms

HSPK 21, HsPK21, NEK2, NEK2A, NimA like protein kinase 1, NimA related protein kinase 2, NLK1

验证数据展示

  • IF Staining of HeLa using CL488-24171

    IF Staining of HeLa using CL488-24171

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 NEK2 antibody (CL488-24171) at dilution of 1:400.

经过测试的应用

Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy NEK2 Ready-To-Use IHC Kit. NEK2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 24171-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-24171 targets NEK2 in IF/ICC applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, mouse
Immunogen NEK2 fusion protein Ag21476 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name NIMA (never in mitosis gene a)-related kinase 2
Synonyms HSPK 21, HsPK21, NEK2, NEK2A, NimA like protein kinase 1, NimA related protein kinase 2, NLK1
Calculated Molecular Weight 52 kDa
Observed Molecular Weight 48 kDa
GenBank Accession NumberBC043502
Gene Symbol NEK2
Gene ID (NCBI) 4751
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP51955
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 NEK2 antibody CL488-24171Download protocol
Standard Protocols
Click here to view our Standard Protocols