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Species Reactivity
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Species Reactivity
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Conjugate
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验证数据展示

  • IHC staining of human lymphoma using 11192-1-AP (same clone as 11192-1-PBS)

    IHC staining of human lymphoma using 11192-1-AP (same clone as 11192-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 11192-1-AP (NEK9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11192-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NEK9 antibody included
  • WB analysis using 11192-1-AP (same clone as 11192-1-PBS)

    WB analysis using 11192-1-AP (same clone as 11192-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11192-1-AP (NEK9 antibody) at dilution of 1:4500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11192-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 11192-1-AP (same clone as 11192-1-PBS)

    IP experiment of HeLa using 11192-1-AP (same clone as 11192-1-PBS)

    IP result of anti-NEK9 (IP:11192-1-AP, 4ug; Detection:11192-1-AP 1:1000) with HeLa cells lysate 1880 ug. This data was developed using the same antibody clone with 11192-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 11192-1-AP (same clone as 11192-1-PBS)

    IF Staining of HepG2 using 11192-1-AP (same clone as 11192-1-PBS)

    Immunofluorescent analysis of HepG2 cells, using NEK9 antibody 11192-1-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 11192-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 11192-1-AP (same clone as 11192-1-PBS)

    IF Staining of HepG2 using 11192-1-AP (same clone as 11192-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NEK9 antibody (11192-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11192-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 11192-1-AP (same clone as 11192-1-PBS)

    FC experiment of HeLa using 11192-1-AP (same clone as 11192-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human NEK9 (11192-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug x. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 11192-1-PBS in a different storage buffer formulation.

NEK9 Polyclonal antibody, PBS Only

NEK9 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA
Cat No. 11192-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy NEK9 Ready-To-Use IHC Kit. NEK9 primary antibody included.

产品信息

11192-1-PBS targets NEK9 in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 NEK9 fusion protein Ag1678 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 NIMA (never in mitosis gene a)- related kinase 9
别名
计算分子量 107 kDa
观测分子量 120 kDa
GenBank蛋白编号BC009336
基因名称 NEK9
Gene ID (NCBI) 91754
RRIDAB_2150800
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8TD19
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NEK9 is also named as KIAA1995, NEK8, NERCC and belongs to the NEK Ser/Thr protein kinase family. It is originally identified as αβ-casein kinase that associates with a putative substrate Bicd2. It is also independently identified as a Nek6- and Ran GTPase-binding protein under a different name, Nercc1(PMID:14660563). Nek9, together with the highly similar (80% identical) Nek6 and Nek7, form a signaling module that is activated during mitosis and involved in the regulation of the mitotic spindle. The endogenous Nek9 (with predicted molecular mass of 120 kDa) has an apparent molecular mass of 600 kDa, also compatible with a tetramer(PMID:21454704).