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NEK9 Polyclonal antibody, PBS Only

NEK9 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 11192-1-PBS

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Synonyms

验证数据展示

  • IHC staining of human lymphoma using 11192-1-AP (same clone as 11192-1-PBS)

    IHC staining of human lymphoma using 11192-1-AP (same clone as 11192-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 11192-1-AP (NEK9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11192-1-PBS in a different storage buffer formulation.

  • WB analysis using 11192-1-AP (same clone as 11192-1-PBS)

    WB analysis using 11192-1-AP (same clone as 11192-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11192-1-AP (NEK9 antibody) at dilution of 1:4500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11192-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 11192-1-AP (same clone as 11192-1-PBS)

    IP experiment of HeLa using 11192-1-AP (same clone as 11192-1-PBS)

    IP result of anti-NEK9 (IP:11192-1-AP, 4ug; Detection:11192-1-AP 1:1000) with HeLa cells lysate 1880 ug. This data was developed using the same antibody clone with 11192-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 11192-1-AP (same clone as 11192-1-PBS)

    IF Staining of HepG2 using 11192-1-AP (same clone as 11192-1-PBS)

    Immunofluorescent analysis of HepG2 cells, using NEK9 antibody 11192-1-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 11192-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 11192-1-AP (same clone as 11192-1-PBS)

    IF Staining of HepG2 using 11192-1-AP (same clone as 11192-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NEK9 antibody (11192-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11192-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 11192-1-AP (same clone as 11192-1-PBS)

    FC experiment of HeLa using 11192-1-AP (same clone as 11192-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human NEK9 (11192-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug x. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 11192-1-PBS in a different storage buffer formulation.

产品信息

11192-1-PBS targets NEK9 in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen NEK9 fusion protein Ag1678 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name NIMA (never in mitosis gene a)- related kinase 9
Synonyms
Calculated Molecular Weight 107 kDa
Observed Molecular Weight 120 kDa
GenBank Accession NumberBC009336
Gene Symbol NEK9
Gene ID (NCBI) 91754
RRIDAB_2150800
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8TD19
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NEK9 is also named as KIAA1995, NEK8, NERCC and belongs to the NEK Ser/Thr protein kinase family. It is originally identified as αβ-casein kinase that associates with a putative substrate Bicd2. It is also independently identified as a Nek6- and Ran GTPase-binding protein under a different name, Nercc1(PMID:14660563). Nek9, together with the highly similar (80% identical) Nek6 and Nek7, form a signaling module that is activated during mitosis and involved in the regulation of the mitotic spindle. The endogenous Nek9 (with predicted molecular mass of 120 kDa) has an apparent molecular mass of 600 kDa, also compatible with a tetramer(PMID:21454704).