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验证数据展示

  • WB analysis using 83490-2-RR

    WB analysis using 83490-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83490-2-RR (NELF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83490-2-RR

    Affinity and Kinetic Characterization of 83490-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83490-2-RR against Human NELF were performed. The affinity constant is below 1 pM.

NELF Recombinant antibody

NELF Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 83490-2-RR

Print datasheet

CloneNo. 240482G1

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Synonyms

NSMF, NMDA receptor synaptonuclear signaling and neuronal migration factor, Nasal embryonic luteinizing hormone-releasing hormone factor, Nasal embryonic LHRH factor, 240482G1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inCOLO 320 cells, HeLa cells, HepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83490-2-RR targets NELF in WB, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human
Immunogen NELF fusion protein Ag34902 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name nasal embryonic LHRH factor
Synonyms NSMF, NMDA receptor synaptonuclear signaling and neuronal migration factor, Nasal embryonic luteinizing hormone-releasing hormone factor, Nasal embryonic LHRH factor, 240482G1
Calculated Molecular Weight 507 aa, 56 kDa
Observed Molecular Weight 54-60 kDa
GenBank Accession NumberBC016862
Gene Symbol NELF
Gene ID (NCBI) 26012
RRIDAB_3671117
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ6X4W1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NSMF, also named as NELF (Nasal embryonic LHRH factor), couples NMDA-sensitive glutamate receptor signaling to the nucleus and triggers long-lasting changes in the cytoarchitecture of dendrites and spine synapse processes. NELF plays a major role in promoter-proximal pausing, a widespread phenomenon during early transcription elongation.

实验方案

Product Specific Protocols
WB protocol for NELF antibody 83490-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols