验证数据展示
经过测试的应用
Positive WB detected in | COLO 320 cells, HeLa cells, HepG2 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:5000-1:50000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
83490-2-RR targets NELF in WB, ELISA applications and shows reactivity with Human samples.
经测试应用 | WB, ELISA Application Description |
经测试反应性 | Human |
免疫原 | NELF fusion protein Ag34902 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Recombinant |
产品类型 | Antibody |
全称 | nasal embryonic LHRH factor |
别名 | NSMF, NMDA receptor synaptonuclear signaling and neuronal migration factor, Nasal embryonic luteinizing hormone-releasing hormone factor, Nasal embryonic LHRH factor, 240482G1 |
计算分子量 | 507 aa, 56 kDa |
观测分子量 | 54-60 kDa |
GenBank蛋白编号 | BC016862 |
基因名称 | NELF |
Gene ID (NCBI) | 26012 |
RRID | AB_3671117 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | Q6X4W1 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NSMF, also named as NELF (Nasal embryonic LHRH factor), couples NMDA-sensitive glutamate receptor signaling to the nucleus and triggers long-lasting changes in the cytoarchitecture of dendrites and spine synapse processes. NELF plays a major role in promoter-proximal pausing, a widespread phenomenon during early transcription elongation.
实验方案
Product Specific Protocols | |
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WB protocol for NELF antibody 83490-2-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |