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CoraLite® Plus 488-conjugated NEU1 Monoclonal antibody

NEU1 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat, Pig

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1F6D1

Cat no : CL488-67032

Print datasheet

Synonyms

Acetylneuraminyl hydrolase, FLJ93471, G9 sialidase, Lysosomal sialidase, N acetyl alpha neuraminidase 1, NANH, NEU, NEU1, NEURAMINIDASE 1, SIAL1, Sialidase 1

验证数据展示

  • IF Staining of U-251 using CL488-67032

    IF Staining of U-251 using CL488-67032

    Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using CoraLite® Plus 488 NEU1 antibody (CL488-67032, Clone: 1F6D1 ) at dilution of 1:200.

经过测试的应用

Positive IF/ICC detected inU-251 cells
For other applications, we recommend the unconjugated version of this antibody, 67032-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67032 targets NEU1 in IF/ICC applications and shows reactivity with Human, Rat, Pig samples.

Tested Applications IF/ICC Application Description
Tested Reactivity Human, Rat, Pig
Immunogen NEU1 fusion protein Ag27945 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name sialidase 1 (lysosomal sialidase)
Synonyms Acetylneuraminyl hydrolase, FLJ93471, G9 sialidase, Lysosomal sialidase, N acetyl alpha neuraminidase 1, NANH, NEU, NEU1, NEURAMINIDASE 1, SIAL1, Sialidase 1
Calculated Molecular Weight 45 kDa
Observed Molecular Weight 45-48 kDa
GenBank Accession NumberBC011900
Gene Symbol NEU1
Gene ID (NCBI) 4758
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ99519
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NEU1 (lysosomal sialidase) encodes a kind of lysosomal enzyme that could cleave terminal silic acids from glycoproteins or glycolipids to regulate various biological processes by conformational change (PMID:28130415). In the lysosome, NEU1 is one member of a heterotrimeric complex, the others are beta-galactosidase and cathepsin A. The NEU1 is widely expressed in mammalian tissues and involved in lysosomal storage disorder sialidosis, autoimmune diseases and the malignancy and metastasis of cancer cells (PMID:19075514). NUE1 also expresses on the plasma membrane where it modulates several signalling molecules about inflamation, exocytosis, phagocytosis, cell adhesion and proliferation (PMID:21928149). Addition, it is reported that mutation in NEU1 of human could lead to sialidosis (PMID:14517945).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 NEU1 antibody CL488-67032Download protocol
Standard Protocols
Click here to view our Standard Protocols