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验证数据展示

  • IF Staining of PC-12 using 83934-2-RR

    IF Staining of PC-12 using 83934-2-RR

    Immunofluorescent analysis of (4% PFA) fixed PC-12 cells using NEU2 antibody (83934-2-RR, Clone: 240685C12 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • FC experiment of A549 using 83934-2-RR

    FC experiment of A549 using 83934-2-RR

    1x10^6 A549 cells were intracellularly stained with 0.25 ug Neu2 Recombinant Antibody (83934-2-RR, Clone:240685C12) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

NEU2 Recombinant antibody

NEU2 Recombinant Antibody for IF/ICC, FC (Intra), ELISA
Cat No. 83934-2-RR

产品说明书

CloneNo. 240685C12

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

IF/ICC, FC (Intra), ELISA

别名

SIAL2, N-acetyl-alpha-neuraminidase 2, N acetyl alpha neuraminidase 2, EC:3.2.1.18, Cytosolic sialidase

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inPC-12 cells
Positive FC (Intra) detected inA549 cells

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83934-2-RR targets NEU2 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, rat samples.

经测试应用 IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human, rat
免疫原 NEU2 fusion protein Ag17386 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 sialidase 2 (cytosolic sialidase)
别名 SIAL2, N-acetyl-alpha-neuraminidase 2, N acetyl alpha neuraminidase 2, EC:3.2.1.18, Cytosolic sialidase
计算分子量 380 aa, 42 kDa
GenBank蛋白编号BC107053
基因名称 NEU2
Gene ID (NCBI) 4759
RRIDAB_3671516
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ9Y3R4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NEU2, also named as Sialidase 2, neuraminidase 2, catalyzes the hydrolytic cleavage of the terminal sialic acid (N-acetylneuraminic acid, Neu5Ac) of a glycan moiety in the catabolism of glycolipids, glycoproteins and oligosaccharides. NEU2 is localized in the cytosol and plasma membrane and takes part in myoblast and neuronal differentiation. It is almost undetectable under normal physiological conditions, and was found to be involved into apoptotic events in pancreatic ductal adenocarcinoma, one of the most dangerous cancers due to poor diagnosis and prognosis. (PMID: 30439363)

实验方案

Product Specific Protocols
IF protocol for NEU2 antibody 83934-2-RRDownload protocol
FC protocol for NEU2 antibody 83934-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols