CoraLite®594-conjugated NEUROD1 Monoclonal antibody

NEUROD1 Monoclonal Antibody for FC (Intra)
Cat No. CL594-66691

产品说明书

CloneNo. 3E10F1

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

FC (Intra)

NEUROD, Class A basic helix-loop-helix protein 3, bHLHa3, 3E10F1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -


经过测试的应用

Positive FC (Intra) detected inSH-SY5Y cells
For other applications, we recommend the unconjugated version of this antibody, 66691-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66691 targets NEUROD1 in FC (Intra) applications and shows reactivity with human samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human
免疫原 NEUROD1 fusion protein Ag27606 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 neurogenic differentiation 1
别名 NEUROD, Class A basic helix-loop-helix protein 3, bHLHa3, 3E10F1
计算分子量 356 aa, 40 kDa
观测分子量 40-50 kDa
GenBank蛋白编号BC009046
基因名称 NEUROD1
Gene ID (NCBI) 4760
RRIDAB_2923923
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ13562
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NeuroD is a member of the basic helix-loop-helix (bHLH) family of transcription factors. The basic helix-loop-helix (bHLH) proteins are transcription factors that are required for several aspects of development, including cell type determination, terminal differentiation and sex determination. Members of the myogenic determination family, MyoD, myf5, myogenin and MRF4, all have bHLH domains.These proteins function by forming heterodimers with E-proteins and binding to the canonical E-box sequence CANNTG. Neuro D is expressed transiently in a subset of neurons in the central and peripheral nervous systems at the time of their terminal differentiation into mature neurons. Moreover, ectopic expression of Neuro D in Xenopus embryos induces premature differentiation of neuronal precursors and Neuro D can convert presumptive epidermal cells into neurons.The lack of NeuroD in the brain results in severe defects in development. Human mutations have been linked to a number of types of diabetes including type I diabetes mellitus and maturity-onset diabetes of the young. The calculated molecular weight of NEUROD1 is 39 kDa, but the modified NEUROD1 protein is about 45-50 kDa.

实验方案

Product Specific Protocols
FC protocol for CL594 NEUROD1 antibody CL594-66691Download protocol
Standard Protocols
Click here to view our Standard Protocols
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