NF-H/NF200 Monoclonal antibody
NF-H/NF200 Monoclonal Antibody for WB, IHC, IF-P, FC (Intra), ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
human, mouse, rat, pig
Applications
WB, IHC, IF-P, FC (Intra), ELISA and More (1)
Conjugate
Unconjugated
CloneNo.
1A3C7
验证数据展示
经过测试的应用
Positive WB detected in | pig cerebellum tissue, rat brain tissue, mouse brain tissue |
Positive IHC detected in | rat cerebellum tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | rat brain tissue |
Positive FC (Intra) detected in | SH-SY5Y cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:4000-1:16000 |
Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
60331-1-Ig targets NF-H/NF200 in WB, IHC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig samples.
Tested Applications | WB, IHC, IF-P, FC (Intra), ELISA Application Description |
Cited Applications | WB, IHC, IF |
Tested Reactivity | human, mouse, rat, pig |
Cited Reactivity | human, mouse, rat |
Immunogen | NF-H/NF200 fusion protein Ag13517 种属同源性预测 |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Full Name | neurofilament, heavy polypeptide |
Synonyms | NEFH, Neurofilament heavy polypeptide, NF200, NF-H, neurofilament heavy chain |
Calculated Molecular Weight | 112 kDa |
Observed Molecular Weight | 200 kDa |
GenBank Accession Number | BC014185 |
Gene Symbol | NF-H |
Gene ID (NCBI) | 4744 |
RRID | AB_2881440 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P12036 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for NF-H/NF200 antibody 60331-1-Ig | Download protocol |
IHC protocol for NF-H/NF200 antibody 60331-1-Ig | Download protocol |
IF protocol for NF-H/NF200 antibody 60331-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
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Nat Commun Macrophage lineage cells-derived migrasomes activate complement-dependent blood-brain barrier damage in cerebral amyloid angiopathy mouse model | ||
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J Neuroinflammation Apelin alleviated neuroinflammation and promoted endogenous neural stem cell proliferation and differentiation after spinal cord injury in rats. | ||
Biomolecules A Combination of Heavy Metals and Intracellular Pathway Modulators Induces Alzheimer Disease-like Pathologies in Organotypic Brain Slices |