NF45 Monoclonal antibody

NF45 Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 67685-1-Ig

产品说明书

CloneNo. 1A3A2

宿主/亚型

Mouse / IgG2b

种属反应性

Human, mouse, rat and More (1)

应用

WB, IHC, IF/ICC, IP, ELISA and More (2)

ILF2, NF45, PRO3063

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -


经过测试的应用

Positive WB detected inMCF-7 cells, HeLa cells, A431 cells, HepG2 cells, MOLT-4 cells, Jurkat cells, HSC-T6 cells, PC-12 cells, Sp2/0 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman colon cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67685-1-Ig targets NF45 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IF, CoIP
经测试反应性 Human, mouse, rat
文献引用反应性human, mouse
免疫原 NF45 fusion protein Ag30388 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 interleukin enhancer binding factor 2, 45kDa
别名 ILF2, NF45, PRO3063
计算分子量 43 kDa
观测分子量40 kDa
GenBank蛋白编号BC000382
基因名称 ILF2
Gene ID (NCBI) 3608
RRIDAB_2882878
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ12905
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Interleukin-2 enhancer binding factor 2 (ILF2), also named as nuclear factor 45 (NF45), plays important roles in regulating interleukin-2 expression in mammals. It forms a heterodimeric complex with ILF3, which may regulate transcription of the IL2 gene during T-cell activation. It can also promote the formation of stable DNA-dependent protein kinase holoenzyme complexes on DNA [PMID:11739746,17129403].

实验方案

Product Specific Protocols
WB protocol for NF45 antibody 67685-1-IgDownload protocol
IHC protocol for NF45 antibody 67685-1-IgDownload protocol
IF protocol for NF45 antibody 67685-1-IgDownload protocol
IP protocol for NF45 antibody 67685-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB,IF,CoIP

Cell Rep

Virus-induced lncRNA-BTX allows viral replication by regulating intracellular translocation of DHX9 and ILF3 to induce innate escape

Authors - Yang Cao
humanWB,CoIP

J Exp Clin Cancer Res

LINC00571 drives tricarboxylic acid cycle metabolism in triple-negative breast cancer through HNRNPK/ILF2/IDH2 axis

Authors - Zihan Xi
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