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CoraLite® Plus 488-conjugated NF45 Monoclonal antibody

NF45 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1A3A2

Cat no : CL488-67685

Print datasheet

Synonyms

ILF2, PRO3063, Nuclear factor of activated T-cells 45 kDa, Interleukin enhancer-binding factor 2, 1A3A2

验证数据展示

  • IF Staining of HepG2 using CL488-67685

    IF Staining of HepG2 using CL488-67685

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 NF45 antibody (CL488-67685, Clone: 1A3A2 ) at dilution of 1:200.

  • FC experiment of HepG2 using CL488-67685

    FC experiment of HepG2 using CL488-67685

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human NF45 (CL488-67685, Clone:1A3A2) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 67685-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67685 targets NF45 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen NF45 fusion protein Ag30388 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name interleukin enhancer binding factor 2, 45kDa
Synonyms ILF2, PRO3063, Nuclear factor of activated T-cells 45 kDa, Interleukin enhancer-binding factor 2, 1A3A2
Calculated Molecular Weight 43 kDa
Observed Molecular Weight40 kDa
GenBank Accession NumberBC000382
Gene Symbol ILF2
Gene ID (NCBI) 3608
RRIDAB_2919519
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ12905
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Interleukin-2 enhancer binding factor 2 (ILF2), also named as nuclear factor 45 (NF45), plays important roles in regulating interleukin-2 expression in mammals. It forms a heterodimeric complex with ILF3, which may regulate transcription of the IL2 gene during T-cell activation. It can also promote the formation of stable DNA-dependent protein kinase holoenzyme complexes on DNA [PMID:11739746,17129403].

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 NF45 antibody CL488-67685Download protocol
FC protocol for CL Plus 488 NF45 antibody CL488-67685Download protocol
Standard Protocols
Click here to view our Standard Protocols