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NF90/ILF3 Polyclonal antibody

NF90/ILF3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 19887-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA and More (3)

ILF3, M phase phosphoprotein 4, IL-F3, DRBP76, DRBF

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells, mouse spleen tissue, K-562 cells, Raji cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman cervical cancer tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHela cells
Planning an IHC experiment? We recommend our IHCeasy ILF3 Ready-To-Use IHC Kit. ILF3 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19887-1-AP targets NF90/ILF3 in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC, IF, IP, CoIP, RIP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat
免疫原 NF90/ILF3 fusion protein Ag13707 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 interleukin enhancer binding factor 3, 90kDa
别名 ILF3, M phase phosphoprotein 4, IL-F3, DRBP76, DRBF
计算分子量 894 aa, 95 kDa
观测分子量 90 kDa, 110 kDa
GenBank蛋白编号BC064836
基因名称 ILF3
Gene ID (NCBI) 3609
RRIDAB_10666431
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ12906
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Nuclear factor of activated T cells (NFAT)is a transcription factor required for T-cell expression of interleukin-2 (IL2). NFAT binds to a sequence known as the antigen receptor response element-2 (ARRE2) in the IL2 enhancer. NFAT contains two subunits, one is ILF3. ILF3 can facilitate double-standed RNA-regulated gene expression at the level of post-transcription. It binds to and inhibits coding sequences of acid beta-glucosidase and other mRNAs and functions at the initiation phase of GCase mRNA translation, probably by inhibiting its binding to polysomes. ILF3 exists main two isoforms NFAR-1 (90 kDa) and -2 (110 kDa). NFAR-1 and -2 each contain two DRBDs in their C-terminus regions and exhibit 98% homology to one another at the amino acid level. NFAR-2 contains an extra 192 amino acids at the C-terminal region that are encoded by an extra three exons present in its corresponding transcript.

实验方案

Product Specific Protocols
WB protocol for NF90/ILF3 antibody 19887-1-APDownload protocol
IHC protocol for NF90/ILF3 antibody 19887-1-APDownload protocol
IF protocol for NF90/ILF3 antibody 19887-1-APDownload protocol
IP protocol for NF90/ILF3 antibody 19887-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell

In vivo structural characterization of the SARS-CoV-2 RNA genome identifies host proteins vulnerable to repurposed drugs.

Authors - Lei Sun
  • KD Validated
humanWB

Nat Commun

PMID: 31792210

Authors - Sunwang Xu
humanWB

EMBO J

Suppression of miR-199a maturation by HuR is crucial for hypoxia-induced glycolytic switch in hepatocellular carcinoma.

Authors - Ling-Fei Zhang
humanWB

Dev Cell

Genome-wide analysis of pseudogenes reveals HBBP1's human-specific essentiality in erythropoiesis and implication in β-thalassemia.

Authors - Yanni Ma
mouseRIP

Cancer Res

CircFOXK2 Promotes Growth and Metastasis of Pancreatic Ductal Adenocarcinoma by Complexing with RNA Binding Proteins and Sponging MiR-942.

Authors - Chi Hin Wong
humanWB

Nucleic Acids Res

Oxygen-dependent expression of cytochrome c oxidase subunit 4-2 gene expression is mediated by transcription factors RBPJ, CXXC5 and CHCHD2.

Authors - Aras Siddhesh S
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