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验证数据展示

  • WB analysis using 68213-1-Ig

    WB analysis using 68213-1-Ig

    A431 cells were subjected to SDS PAGE followed by western blot with 68213-1-Ig (NF90/ILF3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • WB analysis using 68213-1-Ig

    WB analysis using 68213-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68213-1-Ig (NF90/ILF3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • IHC staining of mouse eye using 68213-1-Ig

    IHC staining of mouse eye using 68213-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 68213-1-Ig (NF90/ILF3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ILF3 antibody included

NF90/ILF3 Monoclonal antibody

NF90/ILF3 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 68213-1-Ig

产品说明书

CloneNo. 2H10G3

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse, rat

应用

WB, IHC, ELISA

别名

CBTF, DRBF, DRBP76, ILF3, M phase phosphoprotein 4, MMP4, MPHOSPH4, MPP4, NF AT 90, NF110, NF90, NFAR, NFAR 1, NFAR2, TCP110, TCP80

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inA431 cells, HSC-T6 cells, LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, NIH/3T3 cells, 4T1 cells
Positive IHC detected inmouse eye tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy ILF3 Ready-To-Use IHC Kit. ILF3 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68213-1-Ig targets NF90/ILF3 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原 NF90/ILF3 fusion protein Ag30868 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 interleukin enhancer binding factor 3, 90kDa
别名 CBTF, DRBF, DRBP76, ILF3, M phase phosphoprotein 4, MMP4, MPHOSPH4, MPP4, NF AT 90, NF110, NF90, NFAR, NFAR 1, NFAR2, TCP110, TCP80
计算分子量 894 aa, 95 kDa
观测分子量90 kDa, 110 kDa
GenBank蛋白编号BC064836
基因名称 ILF3
Gene ID (NCBI) 3609
RRIDAB_2935301
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ12906
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for NF90/ILF3 antibody 68213-1-IgDownload protocol
IHC protocol for NF90/ILF3 antibody 68213-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols