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验证数据展示

  • WB analysis using 68213-1-Ig

    WB analysis using 68213-1-Ig

    A431 cells were subjected to SDS PAGE followed by western blot with 68213-1-Ig (NF90/ILF3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • WB analysis using 68213-1-Ig

    WB analysis using 68213-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68213-1-Ig (NF90/ILF3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • IHC staining of mouse eye using 68213-1-Ig

    IHC staining of mouse eye using 68213-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 68213-1-Ig (NF90/ILF3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ILF3 antibody included

NF90/ILF3 Monoclonal antibody

NF90/ILF3 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 68213-1-Ig

Print datasheet

CloneNo. 2H10G3

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

CBTF, DRBF, DRBP76, ILF3, M phase phosphoprotein 4, MMP4, MPHOSPH4, MPP4, NF AT 90, NF110, NF90, NFAR, NFAR 1, NFAR2, TCP110, TCP80

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive WB detected inA431 cells, HSC-T6 cells, LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, NIH/3T3 cells, 4T1 cells
Positive IHC detected inmouse eye tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy ILF3 Ready-To-Use IHC Kit. ILF3 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68213-1-Ig targets NF90/ILF3 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen NF90/ILF3 fusion protein Ag30868 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name interleukin enhancer binding factor 3, 90kDa
Synonyms CBTF, DRBF, DRBP76, ILF3, M phase phosphoprotein 4, MMP4, MPHOSPH4, MPP4, NF AT 90, NF110, NF90, NFAR, NFAR 1, NFAR2, TCP110, TCP80
Calculated Molecular Weight 894 aa, 95 kDa
Observed Molecular Weight90 kDa, 110 kDa
GenBank Accession NumberBC064836
Gene Symbol ILF3
Gene ID (NCBI) 3609
RRIDAB_2935301
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ12906
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for NF90/ILF3 antibody 68213-1-IgDownload protocol
IHC protocol for NF90/ILF3 antibody 68213-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols