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NFATC3 Monoclonal antibody, PBS Only (Detector)

NFATC3 Monoclonal Antibody for WB, Sandwich ELISA, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, Sandwich ELISA, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1C1H8

Cat No : 66924-1-PBS

Print datasheet

Synonyms

NF AT4, NF ATc3, NFAT4, NFATC3, NFATX

验证数据展示

  • WB analysis using 66924-1-Ig (same clone as 66924-1-PBS)

    WB analysis using 66924-1-Ig (same clone as 66924-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66924-1-Ig (NFATC3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66924-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP50022-1

    Sandwich ELISA standard curve of MP50022-1

    Sandwich ELISA standard curve ofMP50022-1, NFATC3 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66924-2-PBS. Detection antibody: HRP-conjugated 66924-1-PBS. Standard: Ag27306. Range: 0.625-20 ng/mL.

Planning an IHC experiment? We recommend our IHCeasy NFATC3 Ready-To-Use IHC Kit. NFATC3 primary antibody included.

产品信息

66924-1-PBS targets NFATC3 as part of a matched antibody pair:

MP50022-1: 66924-2-PBS capture and 66924-1-PBS detection (validated in Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, Sandwich ELISA, Indirect ELISA Application Description
Tested Reactivity Human
Immunogen NFATC3 fusion protein Ag27306 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Affinity
KD=1.16 x 10-10M
KOff=5.67 x 10-6M
KOn=4.90 x 104M
Full Name nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 3
Synonyms NF AT4, NF ATc3, NFAT4, NFATC3, NFATX
Calculated Molecular Weight 116 kDa
Observed Molecular Weight 160 kDa
GenBank Accession NumberBC001050
Gene Symbol NFATC3
Gene ID (NCBI) 4775
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ12968
Storage Buffer 100% PBS pH 7.3
Storage ConditionsStore at -80°C.

背景介绍

NFAT (nuclear factors of activated T cells) proteins are a family of transcription factors originally identified as mediators of activation of cytokine genes in response to antigenic stimulation of T cells. NFAT proteins also play varied roles in cells outside of the immune system [PMID:11877454]. NFATc3 has specifically been implicated in vasculature development, regulation of smooth muscle contractile phenotype, and modulation of vascular smooth muscle contractility [PMID:11439183,17148444]. The calculated molecular weight of NFATC3 is 115 kDa, but the post-modified protein is about 130-170 kDa (PMID: 15728531, PMID: 15857835)