• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 66924-1-Ig (same clone as 66924-1-PBS)

    WB analysis using 66924-1-Ig (same clone as 66924-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66924-1-Ig (NFATC3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66924-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP50022-1

    Sandwich ELISA standard curve of MP50022-1

    Sandwich ELISA standard curve ofMP50022-1, NFATC3 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66924-2-PBS. Detection antibody: HRP-conjugated 66924-1-PBS. Standard: Ag27306. Range: 0.625-20 ng/mL.

NFATC3 Monoclonal antibody, PBS Only (Detector)

NFATC3 Monoclonal Antibody for WB, Sandwich ELISA, Indirect ELISA
Cat No. 66924-1-PBS

产品说明书

CloneNo. 1C1H8

宿主/亚型

Mouse / IgG1

种属反应性

Human

应用

WB, Sandwich ELISA, Indirect ELISA

别名

NF AT4, NF ATc3, NFAT4, NFATC3, NFATX

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

Planning an IHC experiment? We recommend our IHCeasy NFATC3 Ready-To-Use IHC Kit. NFATC3 primary antibody included.

产品信息

66924-1-PBS targets NFATC3 as part of a matched antibody pair:

MP50022-1: 66924-2-PBS capture and 66924-1-PBS detection (validated in Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, Sandwich ELISA, Indirect ELISA Application Description
经测试反应性 Human
免疫原 NFATC3 fusion protein Ag27306 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
亲和力
KD=1.16 x 10-10M
KOff=5.67 x 10-6M
KOn=4.90 x 104M
全称 nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 3
别名 NF AT4, NF ATc3, NFAT4, NFATC3, NFATX
计算分子量 116 kDa
观测分子量 160 kDa
GenBank蛋白编号BC001050
基因名称 NFATC3
Gene ID (NCBI) 4775
RRIDAB_2882251
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ12968
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NFAT (nuclear factors of activated T cells) proteins are a family of transcription factors originally identified as mediators of activation of cytokine genes in response to antigenic stimulation of T cells. NFAT proteins also play varied roles in cells outside of the immune system [PMID:11877454]. NFATc3 has specifically been implicated in vasculature development, regulation of smooth muscle contractile phenotype, and modulation of vascular smooth muscle contractility [PMID:11439183,17148444]. The calculated molecular weight of NFATC3 is 115 kDa, but the post-modified protein is about 130-170 kDa (PMID: 15728531, PMID: 15857835)