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验证数据展示

  • WB analysis using 67983-1-Ig

    WB analysis using 67983-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67983-1-Ig (NFIX antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • WB analysis of HeLa using 67983-1-Ig

    WB analysis of HeLa using 67983-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 67983-1-Ig (NFIX antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 67983-1-Ig

    IHC staining of mouse brain using 67983-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67983-1-Ig (NFIX antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NFIX antibody included
  • IHC staining of mouse brain using 67983-1-Ig

    IHC staining of mouse brain using 67983-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67983-1-Ig (NFIX antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NFIX antibody included
  • IHC staining of rat brain using 67983-1-Ig

    IHC staining of rat brain using 67983-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67983-1-Ig (NFIX antibody) at dilution of 1:1500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NFIX antibody included
  • IHC staining of rat brain using 67983-1-Ig

    IHC staining of rat brain using 67983-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67983-1-Ig (NFIX antibody) at dilution of 1:1500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NFIX antibody included

NFIX Monoclonal antibody

NFIX Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67983-1-Ig
CloneNo. 1E8A1

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat, pig, rabbit

Applications

WB, IHC, ELISA

Synonyms

CTF, NF I/X, NF1 X, NF1A, NFI X, NFIX, Nuclear factor 1 X type, Nuclear factor 1/X, Nuclear factor I/X, TGGCA binding protein

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inpig brain tissue, A549 cells, HeLa cells, rat testis tissue, rat brain tissue, rabbit brain tissue, mouse brain tissue
Positive IHC detected inmouse brain tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy NFIX Ready-To-Use IHC Kit. NFIX primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67983-1-Ig targets NFIX in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat, pig, rabbit samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity Human, mouse, rat, pig, rabbit
Immunogen NFIX fusion protein Ag31447 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name nuclear factor I/X (CCAAT-binding transcription factor)
Synonyms CTF, NF I/X, NF1 X, NF1A, NFI X, NFIX, Nuclear factor 1 X type, Nuclear factor 1/X, Nuclear factor I/X, TGGCA binding protein
Calculated Molecular Weight 50 kDa
Observed Molecular Weight45kDa, 50kDa
GenBank Accession NumberNM_001271043
Gene Symbol NFIX
Gene ID (NCBI) 4784
RRIDAB_2918732
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ14938
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NFIX, also named CTF, belongs to the CTF/NF-I family. NFIX recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. NFIX is individually capable of activating transcription and replication.

实验方案

Product Specific Protocols
WB protocol for NFIX antibody 67983-1-IgDownload protocol
IHC protocol for NFIX antibody 67983-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols