验证数据展示
经过测试的应用
Positive WB detected in | pig brain tissue, A549 cells, HeLa cells, rat testis tissue, rat brain tissue, rabbit brain tissue, mouse brain tissue |
Positive IHC detected in | mouse brain tissue, rat brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:2000-1:10000 |
Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
67983-1-Ig targets NFIX in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat, pig, rabbit samples.
Tested Applications | WB, IHC, ELISA Application Description |
Tested Reactivity | Human, mouse, rat, pig, rabbit |
Immunogen | NFIX fusion protein Ag31447 种属同源性预测 |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Full Name | nuclear factor I/X (CCAAT-binding transcription factor) |
Synonyms | CTF, NF I/X, NF1 X, NF1A, NFI X, NFIX, Nuclear factor 1 X type, Nuclear factor 1/X, Nuclear factor I/X, TGGCA binding protein |
Calculated Molecular Weight | 50 kDa |
Observed Molecular Weight | 45kDa, 50kDa |
GenBank Accession Number | NM_001271043 |
Gene Symbol | NFIX |
Gene ID (NCBI) | 4784 |
RRID | AB_2918732 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | Q14938 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NFIX, also named CTF, belongs to the CTF/NF-I family. NFIX recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. NFIX is individually capable of activating transcription and replication.
实验方案
Product Specific Protocols | |
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WB protocol for NFIX antibody 67983-1-Ig | Download protocol |
IHC protocol for NFIX antibody 67983-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |