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验证数据展示

  • WB analysis using 67983-1-Ig

    WB analysis using 67983-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67983-1-Ig (NFIX antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • WB analysis of HeLa using 67983-1-Ig

    WB analysis of HeLa using 67983-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 67983-1-Ig (NFIX antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 67983-1-Ig

    IHC staining of mouse brain using 67983-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67983-1-Ig (NFIX antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NFIX antibody included
  • IHC staining of mouse brain using 67983-1-Ig

    IHC staining of mouse brain using 67983-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67983-1-Ig (NFIX antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NFIX antibody included
  • IHC staining of rat brain using 67983-1-Ig

    IHC staining of rat brain using 67983-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67983-1-Ig (NFIX antibody) at dilution of 1:1500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NFIX antibody included
  • IHC staining of rat brain using 67983-1-Ig

    IHC staining of rat brain using 67983-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67983-1-Ig (NFIX antibody) at dilution of 1:1500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NFIX antibody included

NFIX Monoclonal antibody

NFIX Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67983-1-Ig

产品说明书

CloneNo. 1E8A1

宿主/亚型

Mouse / IgG2b

种属反应性

Human, mouse, rat, pig, rabbit

应用

WB, IHC, ELISA

别名

CTF, NF I/X, NF1 X, NF1A, NFI X, NFIX, Nuclear factor 1 X type, Nuclear factor 1/X, Nuclear factor I/X, TGGCA binding protein

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inpig brain tissue, A549 cells, HeLa cells, rat testis tissue, rat brain tissue, rabbit brain tissue, mouse brain tissue
Positive IHC detected inmouse brain tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy NFIX Ready-To-Use IHC Kit. NFIX primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67983-1-Ig targets NFIX in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat, pig, rabbit samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human, mouse, rat, pig, rabbit
免疫原 NFIX fusion protein Ag31447 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 nuclear factor I/X (CCAAT-binding transcription factor)
别名 CTF, NF I/X, NF1 X, NF1A, NFI X, NFIX, Nuclear factor 1 X type, Nuclear factor 1/X, Nuclear factor I/X, TGGCA binding protein
计算分子量 50 kDa
观测分子量45kDa, 50kDa
GenBank蛋白编号NM_001271043
基因名称 NFIX
Gene ID (NCBI) 4784
RRIDAB_2918732
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ14938
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NFIX, also named CTF, belongs to the CTF/NF-I family. NFIX recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. NFIX is individually capable of activating transcription and replication.

实验方案

Product Specific Protocols
WB protocol for NFIX antibody 67983-1-IgDownload protocol
IHC protocol for NFIX antibody 67983-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols