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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 67983-1-Ig (same clone as 67983-1-PBS)

    WB analysis using 67983-1-Ig (same clone as 67983-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67983-1-Ig (NFIX antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67983-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 67983-1-Ig (same clone as 67983-1-PBS)

    WB analysis of HeLa using 67983-1-Ig (same clone as 67983-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 67983-1-Ig (NFIX antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67983-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 67983-1-Ig (same clone as 67983-1-PBS)

    IHC staining of mouse brain using 67983-1-Ig (same clone as 67983-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67983-1-Ig (NFIX antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67983-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NFIX antibody included
  • IHC staining of mouse brain using 67983-1-Ig (same clone as 67983-1-PBS)

    IHC staining of mouse brain using 67983-1-Ig (same clone as 67983-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67983-1-Ig (NFIX antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67983-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NFIX antibody included
  • IHC staining of rat brain using 67983-1-Ig (same clone as 67983-1-PBS)

    IHC staining of rat brain using 67983-1-Ig (same clone as 67983-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67983-1-Ig (NFIX antibody) at dilution of 1:1500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67983-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NFIX antibody included
  • IHC staining of rat brain using 67983-1-Ig (same clone as 67983-1-PBS)

    IHC staining of rat brain using 67983-1-Ig (same clone as 67983-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67983-1-Ig (NFIX antibody) at dilution of 1:1500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67983-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NFIX antibody included

NFIX Monoclonal antibody, PBS Only

NFIX Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 67983-1-PBS
CloneNo. 1E8A1

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat, pig, rabbit

Applications

WB, IHC, Indirect ELISA

Synonyms

CTF, NF I/X, NF1 X, NF1A, NFI X, NFIX, Nuclear factor 1 X type, Nuclear factor 1/X, Nuclear factor I/X, TGGCA binding protein

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy NFIX Ready-To-Use IHC Kit. NFIX primary antibody included.

产品信息

67983-1-PBS targets NFIX in WB, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat, pig, rabbit samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity Human, mouse, rat, pig, rabbit
Immunogen NFIX fusion protein Ag31447 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name nuclear factor I/X (CCAAT-binding transcription factor)
Synonyms CTF, NF I/X, NF1 X, NF1A, NFI X, NFIX, Nuclear factor 1 X type, Nuclear factor 1/X, Nuclear factor I/X, TGGCA binding protein
Calculated Molecular Weight 50 kDa
Observed Molecular Weight45kDa, 50kDa
GenBank Accession NumberNM_001271043
Gene Symbol NFIX
Gene ID (NCBI) 4784
RRIDAB_2918732
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ14938
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NFIX, also named CTF, belongs to the CTF/NF-I family. NFIX recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. NFIX is individually capable of activating transcription and replication.