CoraLite® Plus 488-conjugated NFKB2,p100 Recombinant antibody

NFKB2,p100 Uni-rAbTM Recombinant Antibody for IF/ICC
Cat No. CL488-84022-6

产品说明书

CloneNo. 241126F1

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC

NFKB2, LYT10, LYT 10, H2TF1, DNA-binding factor KBF2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -


经过测试的应用

Positive IF/ICC detected inU2OS cells
Planning an IHC experiment? We recommend our IHCeasy NFKB2 Ready-To-Use IHC Kit. NFKB2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 84022-6-PBS

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-84022-6 targets NFKB2,p100 in IF/ICC applications and shows reactivity with human samples.

经测试应用 IF/ICC Application Description
经测试反应性 human
免疫原 NFKB2,p100 fusion protein Ag0662 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 (p49/p100)
别名 NFKB2, LYT10, LYT 10, H2TF1, DNA-binding factor KBF2
计算分子量 97 kDa
观测分子量 100-110 kDa
GenBank蛋白编号BC002844
基因名称 NFKB2
Gene ID (NCBI) 4791
ENSEMBL Gene IDENSG00000077150
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ00653
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. NFKB2 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p100 and generation of p52 by a cotranslational processing. The proteasome-mediated process ensures the production of both p52 and p100 and preserves their independent function. P52 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. P52 and p100 are respectively the minor and major form; the processing of p100 being relatively poor. Isoform p49 is a subunit of the NF-kappa-B protein complex, which stimulates the HIV enhancer in synergy with p65. This antibody can bind p100 isoform of NFKB2 spefically, but not p52 isoform.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 NFKB2,p100 antibody CL488-84022-6Download protocol
Standard Protocols
Click here to view our Standard Protocols
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