CoraLite® Plus 488-conjugated NFKB2,p100 Recombinant antibody

NFKB2,p100 Uni-rAbTM Recombinant Antibody for IF/ICC
Cat No. CL488-84022-6

Print datasheet

CloneNo. 241126F1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

NFKB2, LYT10, LYT 10, H2TF1, DNA-binding factor KBF2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -


经过测试的应用

Positive IF/ICC detected inU2OS cells
Planning an IHC experiment? We recommend our IHCeasy NFKB2 Ready-To-Use IHC Kit. NFKB2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 84022-6-PBS

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-84022-6 targets NFKB2,p100 in IF/ICC applications and shows reactivity with human samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human
Immunogen NFKB2,p100 fusion protein Ag0662 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 (p49/p100)
Synonyms NFKB2, LYT10, LYT 10, H2TF1, DNA-binding factor KBF2
Calculated Molecular Weight 97 kDa
Observed Molecular Weight 100-110 kDa
GenBank Accession NumberBC002844
Gene Symbol NFKB2
Gene ID (NCBI) 4791
ENSEMBL Gene IDENSG00000077150
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ00653
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. NFKB2 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p100 and generation of p52 by a cotranslational processing. The proteasome-mediated process ensures the production of both p52 and p100 and preserves their independent function. P52 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. P52 and p100 are respectively the minor and major form; the processing of p100 being relatively poor. Isoform p49 is a subunit of the NF-kappa-B protein complex, which stimulates the HIV enhancer in synergy with p65. This antibody can bind p100 isoform of NFKB2 spefically, but not p52 isoform.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 NFKB2,p100 antibody CL488-84022-6Download protocol
Standard Protocols
Click here to view our Standard Protocols
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