验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, rat brain tissue, mouse stomach tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
25105-1-AP targets NHE2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | NHE2 fusion protein Ag18801 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | solute carrier family 9 (sodium/hydrogen exchanger), member 2 |
| 别名 | Na(+)/H(+) exchanger 2, NHE 2, NHE2, NHE2/SLC9A2, SLC9A2, Sodium/hydrogen exchanger 2 |
| 计算分子量 | 812 aa, 92 kDa |
| 观测分子量 | 70 kDa |
| GenBank蛋白编号 | BC136377 |
| 基因名称 | NHE2/SLC9A2 |
| Gene ID (NCBI) | 6549 |
| RRID | AB_3085771 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9UBY0 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NHE2, also known as SLC9A2, is a protein-coding gene mainly at the top of gastrointestinal epithelial cells. It mainly encodes a member of the sodium-hydrogen exchanger (NHE) protein family, which is localized to the apical membrane and is easily degraded by lysosomes, resulting in a short half-life. NHE2 is involved in sodium ion transport by exchanging intracellular hydrogen ions into external sodium ions, and helps in the regulation of cell pH and volume to eliminate acids produced by active metabolism or against adverse environmental conditions. NHE2 is widely distributed in tissues of the gastrointestinal tract, kidney, heart, testes, uterus, and adrenal glands (PMID: 37688782). The observed molecular weight of NHE2 is 70 kDa, which might be the unmodified form of NHE2 (PMID: 10444453, 10666043).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NHE2 antibody 25105-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |