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NIFK Polyclonal antibody, PBS Only

NIFK Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

Cat no : 12615-1-PBS

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Synonyms

验证数据展示

  • WB analysis using 12615-1-AP (same clone as 12615-1-PBS)

    WB analysis using 12615-1-AP (same clone as 12615-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12615-1-AP (NIFK antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12615-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 12615-1-AP (same clone as 12615-1-PBS)

    IF Staining of MCF-7 using 12615-1-AP (same clone as 12615-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using 12615-1-AP (NIFK antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12615-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 12615-1-AP (same clone as 12615-1-PBS)

    IHC staining of human liver cancer using 12615-1-AP (same clone as 12615-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12615-1-AP (NIFK antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12615-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 12615-1-AP (same clone as 12615-1-PBS)

    IHC staining of human liver cancer using 12615-1-AP (same clone as 12615-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12615-1-AP (NIFK antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12615-1-PBS in a different storage buffer formulation.

  • FC experiment of MCF-7 using 12615-1-AP (same clone as 12615-1-PBS)

    FC experiment of MCF-7 using 12615-1-AP (same clone as 12615-1-PBS)

    1x10^6 MCF-7 cells were intracellularly stained with 0.4 ug NIFK Polyclonal antibody (12615-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 12615-1-PBS in a different storage buffer formulation.

产品信息

12615-1-PBS targets NIFK in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human
Immunogen NIFK fusion protein Ag3284 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name MKI67 (FHA domain) interacting nucleolar phosphoprotein
Synonyms
Calculated Molecular Weight 293 aa, 34 kDa
Observed Molecular Weight 34 kDa
GenBank Accession NumberBC024238
Gene Symbol NIFK
Gene ID (NCBI) 84365
RRIDAB_2142384
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BYG3
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C