验证数据展示
经过测试的应用
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human tonsillitis tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66202-1-Ig targets NINJ2 in IHC, IF-P, ELISA applications and shows reactivity with human samples.
| 经测试应用 | IHC, IF-P, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | NINJ2 fusion protein Ag5343 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | ninjurin 2 |
| 别名 | Nerve injury induced protein 2, NINJ2, ninjurin 2 |
| 计算分子量 | 142 aa, 16 kDa |
| GenBank蛋白编号 | BC057766 |
| 基因名称 | NINJ2 |
| Gene ID (NCBI) | 4815 |
| RRID | AB_2881593 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q9NZG7 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NINJ2 (Ninjurin2) is a member of the ninjurin family of adhesion molecules which mediate cell-to-cell and cell-to-extracellular matrix interactions during development, differentiation, and regeneration of the peripheral nervous system. The gene encodes NINJ2 is located on chromosome 12p13. NINJ2 mRNA is widely expressed in adult human tissues, with highest level in bone marrow, followed by peripheral leukocytes, lung, and lymph nodes. In the peripheral nervous system, NINJ2 is expressed constitutively in mature sensory and enteric neurons. The expression of NINJ2 is upregulated after nerve injury in Schwann cells, suggesting that it may promote nerve regeneration. It may also play an important role in the pathogenesis of inflammatory disorder. (PMID: 10627596)
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for NINJ2 antibody 66202-1-Ig | Download protocol |
| IF protocol for NINJ2 antibody 66202-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |