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NIT2 Monoclonal antibody, PBS Only

NIT2 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1E5B1

Cat no : 68169-1-PBS

Print datasheet

Synonyms

NIT2, nitrilase family, member 2, Nitrilase homolog 2, Omega amidase NIT2

验证数据展示

  • WB analysis using 68169-1-Ig (same clone as 68169-1-PBS)

    WB analysis using 68169-1-Ig (same clone as 68169-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68169-1-Ig (NIT2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68169-1-PBS in a different storage buffer formulation.

  • WB analysis of pig liver using 68169-1-Ig (same clone as 68169-1-PBS)

    WB analysis of pig liver using 68169-1-Ig (same clone as 68169-1-PBS)

    pig liver tissue were subjected to SDS PAGE followed by western blot with 68169-1-Ig (NIT2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68169-1-PBS in a different storage buffer formulation.

  • WB analysis of rat liver using 68169-1-Ig (same clone as 68169-1-PBS)

    WB analysis of rat liver using 68169-1-Ig (same clone as 68169-1-PBS)

    rat liver tissue were subjected to SDS PAGE followed by western blot with 68169-1-Ig (NIT2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68169-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 68169-1-Ig (same clone as 68169-1-PBS)

    IHC staining of human kidney using 68169-1-Ig (same clone as 68169-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 68169-1-Ig (NIT2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68169-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 68169-1-Ig (same clone as 68169-1-PBS)

    IHC staining of human kidney using 68169-1-Ig (same clone as 68169-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 68169-1-Ig (NIT2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68169-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 68169-1-Ig (same clone as 68169-1-PBS)

    IHC staining of human kidney using 68169-1-Ig (same clone as 68169-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 68169-1-Ig (NIT2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68169-1-PBS in a different storage buffer formulation.

  • IF Staining of U-251 using 68169-1-Ig (same clone as 68169-1-PBS)

    IF Staining of U-251 using 68169-1-Ig (same clone as 68169-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using NIT2 antibody (68169-1-Ig, Clone: 1E5B1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68169-1-PBS in a different storage buffer formulation.

  • IF Staining of U-251 using 68169-1-Ig (same clone as 68169-1-PBS)

    IF Staining of U-251 using 68169-1-Ig (same clone as 68169-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using NIT2 antibody (68169-1-Ig, Clone: 1E5B1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68169-1-PBS in a different storage buffer formulation.

产品信息

68169-1-PBS targets NIT2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity Human, mouse, rat, pig
Immunogen NIT2 fusion protein Ag9889 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name nitrilase family, member 2
Synonyms NIT2, nitrilase family, member 2, Nitrilase homolog 2, Omega amidase NIT2
Calculated Molecular Weight 276 aa, 31 kDa
Observed Molecular Weight31 kDa
GenBank Accession NumberBC020620
Gene Symbol NIT2
Gene ID (NCBI) 56954
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9NQR4
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NIT2 belongs to a branch of the nitrilase superfamily of enzymes that cleave carbon-nitrogen bonds. NIT2 functions as an omega-amidase and catalyzes hydrolysis of alpha-ketoglutaramate, forming alpha-ketoglutarate and ammonia. This reaction is also functionally coupled with a subset of transaminases that reaminate the keto acid analogs of some essential amino acids, most particularly methionine and phenylalanine.