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Host
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Species Reactivity
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Species Reactivity
0
Conjugate
0
Conjugate
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Compatible Species
0
Conjugate
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验证数据展示

  • WB analysis using 68169-1-Ig (same clone as 68169-1-PBS)

    WB analysis using 68169-1-Ig (same clone as 68169-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68169-1-Ig (NIT2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68169-1-PBS in a different storage buffer formulation.

  • WB analysis of pig liver using 68169-1-Ig (same clone as 68169-1-PBS)

    WB analysis of pig liver using 68169-1-Ig (same clone as 68169-1-PBS)

    pig liver tissue were subjected to SDS PAGE followed by western blot with 68169-1-Ig (NIT2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68169-1-PBS in a different storage buffer formulation.

  • WB analysis of rat liver using 68169-1-Ig (same clone as 68169-1-PBS)

    WB analysis of rat liver using 68169-1-Ig (same clone as 68169-1-PBS)

    rat liver tissue were subjected to SDS PAGE followed by western blot with 68169-1-Ig (NIT2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68169-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 68169-1-Ig (same clone as 68169-1-PBS)

    IHC staining of human kidney using 68169-1-Ig (same clone as 68169-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 68169-1-Ig (NIT2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68169-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 68169-1-Ig (same clone as 68169-1-PBS)

    IHC staining of human kidney using 68169-1-Ig (same clone as 68169-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 68169-1-Ig (NIT2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68169-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 68169-1-Ig (same clone as 68169-1-PBS)

    IHC staining of human kidney using 68169-1-Ig (same clone as 68169-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 68169-1-Ig (NIT2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68169-1-PBS in a different storage buffer formulation.

  • IF Staining of U-251 using 68169-1-Ig (same clone as 68169-1-PBS)

    IF Staining of U-251 using 68169-1-Ig (same clone as 68169-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using NIT2 antibody (68169-1-Ig, Clone: 1E5B1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68169-1-PBS in a different storage buffer formulation.

  • IF Staining of U-251 using 68169-1-Ig (same clone as 68169-1-PBS)

    IF Staining of U-251 using 68169-1-Ig (same clone as 68169-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using NIT2 antibody (68169-1-Ig, Clone: 1E5B1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68169-1-PBS in a different storage buffer formulation.

NIT2 Monoclonal antibody, PBS Only

NIT2 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 68169-1-PBS

产品说明书

CloneNo. 1E5B1

宿主/亚型

Mouse / IgG2b

种属反应性

Human, mouse, rat, pig

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

NIT2, nitrilase family, member 2, Nitrilase homolog 2, Omega amidase NIT2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

68169-1-PBS targets NIT2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 Human, mouse, rat, pig
免疫原 NIT2 fusion protein Ag9889 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 nitrilase family, member 2
别名 NIT2, nitrilase family, member 2, Nitrilase homolog 2, Omega amidase NIT2
计算分子量 276 aa, 31 kDa
观测分子量31 kDa
GenBank蛋白编号BC020620
基因名称 NIT2
Gene ID (NCBI) 56954
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9NQR4
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NIT2 belongs to a branch of the nitrilase superfamily of enzymes that cleave carbon-nitrogen bonds. NIT2 functions as an omega-amidase and catalyzes hydrolysis of alpha-ketoglutaramate, forming alpha-ketoglutarate and ammonia. This reaction is also functionally coupled with a subset of transaminases that reaminate the keto acid analogs of some essential amino acids, most particularly methionine and phenylalanine.