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验证数据展示

  • WB analysis using 68169-1-Ig

    WB analysis using 68169-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68169-1-Ig (NIT2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis of pig liver using 68169-1-Ig

    WB analysis of pig liver using 68169-1-Ig

    pig liver tissue were subjected to SDS PAGE followed by western blot with 68169-1-Ig (NIT2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis of rat liver using 68169-1-Ig

    WB analysis of rat liver using 68169-1-Ig

    rat liver tissue were subjected to SDS PAGE followed by western blot with 68169-1-Ig (NIT2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 68169-1-Ig

    IHC staining of human kidney using 68169-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 68169-1-Ig (NIT2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human kidney using 68169-1-Ig

    IHC staining of human kidney using 68169-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 68169-1-Ig (NIT2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human kidney using 68169-1-Ig

    IHC staining of human kidney using 68169-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 68169-1-Ig (NIT2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of U-251 using 68169-1-Ig

    IF Staining of U-251 using 68169-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using NIT2 antibody (68169-1-Ig, Clone: 1E5B1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

  • IF Staining of U-251 using 68169-1-Ig

    IF Staining of U-251 using 68169-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using NIT2 antibody (68169-1-Ig, Clone: 1E5B1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

NIT2 Monoclonal antibody

NIT2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 68169-1-Ig

产品说明书

CloneNo. 1E5B1
引用文献(1)

宿主/亚型

Mouse / IgG2b

种属反应性

Human, mouse, rat, pig and More (1)

应用

WB, IHC, IF/ICC, ELISA

别名

NIT2, nitrilase family, member 2, Nitrilase homolog 2, Omega amidase NIT2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, pig liver tissue, mouse liver tissue, HepG2 cells, Jurkat cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU-251 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

68169-1-Ig targets NIT2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 Human, mouse, rat, pig
文献引用反应性human
免疫原 NIT2 fusion protein Ag9889 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 nitrilase family, member 2
别名 NIT2, nitrilase family, member 2, Nitrilase homolog 2, Omega amidase NIT2
计算分子量 276 aa, 31 kDa
观测分子量31 kDa
GenBank蛋白编号BC020620
基因名称 NIT2
Gene ID (NCBI) 56954
RRIDAB_2923685
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9NQR4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NIT2 belongs to a branch of the nitrilase superfamily of enzymes that cleave carbon-nitrogen bonds. NIT2 functions as an omega-amidase and catalyzes hydrolysis of alpha-ketoglutaramate, forming alpha-ketoglutarate and ammonia. This reaction is also functionally coupled with a subset of transaminases that reaminate the keto acid analogs of some essential amino acids, most particularly methionine and phenylalanine.

实验方案

Product Specific Protocols
WB protocol for NIT2 antibody 68169-1-IgDownload protocol
IHC protocol for NIT2 antibody 68169-1-IgDownload protocol
IF protocol for NIT2 antibody 68169-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Commun Biol

Integrating apaQTL and eQTL analysis identifies a potential causal variant associated with lung adenocarcinoma risk in the Chinese population

Authors - Huiwen Xu
View Article