验证数据展示
经过测试的应用
| Positive IF/ICC detected in | U-251 cells |
For other applications, we recommend the unconjugated version of this antibody, 68169-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-68169 targets NIT2 in IF/ICC applications and shows reactivity with Human, mouse, rat, pig samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | Human, mouse, rat, pig |
| 免疫原 | NIT2 fusion protein Ag9889 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | nitrilase family, member 2 |
| 别名 | NIT2, nitrilase family, member 2, Nitrilase homolog 2, Omega amidase NIT2 |
| 计算分子量 | 276 aa, 31 kDa |
| 观测分子量 | 31 kDa |
| GenBank蛋白编号 | BC020620 |
| 基因名称 | NIT2 |
| Gene ID (NCBI) | 56954 |
| RRID | AB_3084428 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q9NQR4 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NIT2 belongs to a branch of the nitrilase superfamily of enzymes that cleave carbon-nitrogen bonds. NIT2 functions as an omega-amidase and catalyzes hydrolysis of alpha-ketoglutaramate, forming alpha-ketoglutarate and ammonia. This reaction is also functionally coupled with a subset of transaminases that reaminate the keto acid analogs of some essential amino acids, most particularly methionine and phenylalanine.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 NIT2 antibody CL488-68169 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |