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Anti-Mouse NK1.1 (CD161) (PK136)

NK1.1 (CD161) Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG2a, kappa

Reactivity

Mouse

Applications

FC

Conjugate

Unconjugated

CloneNo.

PK136

Cat no : 65138-1-Ig

Print datasheet

Synonyms

CD161, Klrb1a, Ly55a, NK1.1, NK1.1 (CD161), NKR P1A, Nkrp1 a, NKRP12, Nkrp1a

验证数据展示

  • FC experiment of C57BL/6 mouse splenocytes using 65138-1-Ig

    FC experiment of C57BL/6 mouse splenocytes using 65138-1-Ig

    1X10^6 C57BL/6 mouse splenocytes were surface co-stained with APC Anti-Mouse CD49b (DX5) and 0.5 ug Anti-Mouse NK1.1 (CD161) (65138-1-Ig, Clone:PK136) and FITC anti-Mouse IgG2a Antibody at dilution 1:100, or 0.5 ug Mouse IgG2a Isotype Control (C1.18.4) and FITC anti-Mouse IgG2a Antibody at dilution 1:100. Cells were not fixed.

  • FC experiment of C57BL/6 mouse splenocytes using 65138-1-Ig

    FC experiment of C57BL/6 mouse splenocytes using 65138-1-Ig

    1X10^6 C57BL/6 mouse splenocytes were surface stained with 0.5 ug Anti-Mouse NK1.1 (CD161) (65138-1-Ig, Clone:PK136) and FITC anti-Mouse IgG2a Antibody at dilution 1:100, or 0.5 ug Mouse IgG2a Isotype Control (C1.18.4) and FITC anti-Mouse IgG2a Antibody at dilution 1:100. Cells were not fixed.

经过测试的应用

Positive FC detected inC57BL/6 mouse splenocytes
Planning an IF experiment? We recommend our CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

65138-1-Ig targets NK1.1 (CD161) in FC applications and shows reactivity with Mouse samples.

Tested Applications FC Application Description
Tested Reactivity Mouse
Immunogen NK-1+ cells from mouse spleen and bone marrow 种属同源性预测
Host / Isotype Mouse / IgG2a, kappa
Class Monoclonal
Type Antibody
Full Name killer cell lectin-like receptor subfamily B member 1A
Synonyms CD161, Klrb1a, Ly55a, NK1.1, NK1.1 (CD161), NKR P1A, Nkrp1 a, NKRP12, Nkrp1a
GenBank Accession NumberBC120707
Gene Symbol NK1.1
Gene ID (NCBI) 17057
RRIDAB_2918425
Conjugate Unconjugated
Form Liquid
Purification MethodAffinity purification
UNIPROT IDP27811
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

背景介绍

NK1.1 (CD161), also known as KLRB1 or NKR-P1A, is a type II transmembrane C-type lectin-like receptor and is expressed on the cell membrane as disulfide-linked homodimer (PMID: 8077657). It is expressed by the majority of NK cells and subsets of peripheral T cells, including both CD4+ and CD8+ T cells, and is expressed preferentially on adult T cells with a "memory" antigenic phenotype (PMID: 8077657; 22566826). Expression of CD161 correlates with the cytotoxic function of CD16+ NK cells, and ligation of CD161 with its ligand LLT1 inhibits NK cell cytotoxicity and cytokine secretion (PMID: 29686665).

实验方案

Product Specific Protocols
FC protocol for NK1.1 (CD161) antibody 65138-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols