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Mouse IgG2a isotype control Monoclonal antibody

Mouse IgG2a isotype control Monoclonal Antibody for WB, FC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

megathura crenulata

Applications

WB, FC, ELISA and More (1)

Conjugate

Unconjugated

CloneNo.

11A1B2

Cat no : 66360-2-Ig

Print datasheet

Synonyms

Mouse IgG2a

验证数据展示

  • WB analysis using 66360-2-Ig

    WB analysis using 66360-2-Ig

    Various lysates (50 μg/lane) were subjected to SDS PAGE followed by western blot with 66360-2-Ig (Mouse IgG2a isotype control antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • FC experiment of human PBMCs using 66360-2-Ig

    FC experiment of human PBMCs using 66360-2-Ig

    1x10^6 human PBMCs were surface stained with 0.5 ug Mouse IgG2a isotype control Monoclonal antibody (66360-2-Ig, Clone:11A1B2) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1) (red), or unstained (blue). Cells were not fixed.

经过测试的应用

Positive WB detected in
Positive FC detected inhuman PBMCs

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Flow Cytometry (FC)FC : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66360-2-Ig targets Mouse IgG2a isotype control in WB, FC , IP, ELISA applications and shows reactivity with megathura crenulata samples.

Tested Applications WB, FC, ELISA Application Description
Cited ApplicationsFC, IP
Tested Reactivity megathura crenulata
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name Mouse IgG2a isotype control
Synonyms Mouse IgG2a
Gene Symbol
Gene ID (NCBI)
RRIDAB_2881739
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

This antibody is used as the isotype control of mouse IgG2a. It can be used as the isotype control in Flow Cytometry, Immuno-Precipitation and other experiments. The immunogen of this antibody is Keyhole Limpet Hemocyanin (KLH).

实验方案

Product Specific Protocols
WB protocol for Mouse IgG2a isotype control antibody 66360-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
FC

Biosens Bioelectron

Spherical nucleic acids-based cascade signal amplification for highly sensitive detection of exosomes.

Authors - Lei Wang
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IP

Nature

CDK11 regulates pre-mRNA splicing by phosphorylation of SF3B1

Authors - Milan Hluchý
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Nat Commun

Generation of nanobodies from transgenic 'LamaMice' lacking an endogenous immunoglobulin repertoire

Authors - Thomas Eden
View Article