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验证数据展示

  • WB analysis using 66360-2-Ig

    WB analysis using 66360-2-Ig

    Various lysates (50 μg/lane) were subjected to SDS PAGE followed by western blot with 66360-2-Ig (Mouse IgG2a isotype control antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • FC experiment of human PBMCs using 66360-2-Ig

    FC experiment of human PBMCs using 66360-2-Ig

    1x10^6 human PBMCs were surface stained with 0.5 ug Mouse IgG2a isotype control Monoclonal antibody (66360-2-Ig, Clone:11A1B2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1) (red), or unstained (blue). Cells were not fixed.

Mouse IgG2a isotype control Monoclonal antibody

Mouse IgG2a isotype control Monoclonal Antibody for WB, FC , ELISA
Cat No. 66360-2-Ig

产品说明书

CloneNo. 11A1B2
引用文献(5)

宿主/亚型

Mouse / IgG2a

种属反应性

megathura crenulata

应用

WB, FC , ELISA and More (1)

别名

IgG2a isotype control, Mouse IgG2a

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected in
Positive FC detected inhuman PBMCs

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Flow Cytometry (FC)FC : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66360-2-Ig targets Mouse IgG2a isotype control in WB, FC , IP, ELISA applications and shows reactivity with megathura crenulata samples.

经测试应用 WB, FC , ELISA Application Description
文献引用应用FC, IP
经测试反应性 megathura crenulata
免疫原

Peptide

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 Mouse IgG2a isotype control
别名 IgG2a isotype control, Mouse IgG2a
基因名称
Gene ID (NCBI)
RRIDAB_2881739
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

This antibody is used as the isotype control of mouse IgG2a. It can be used as the isotype control in Flow Cytometry, Immuno-Precipitation and other experiments. The immunogen of this antibody is Keyhole Limpet Hemocyanin (KLH).

实验方案

Product Specific Protocols
WB protocol for Mouse IgG2a isotype control antibody 66360-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

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Nat Commun

Generation of nanobodies from transgenic 'LamaMice' lacking an endogenous immunoglobulin repertoire

Authors - Thomas Eden
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Plant Biotechnol J

ZmHSFA2B self-regulatory loop is critical for heat tolerance in maize

Authors - Nannan Song
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J Vasc Interv Radiol

Ethiodized Oil Pickering Emulsion for Sustained Release of Anti-PD-L1 Antibodies

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