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验证数据展示

  • IF Staining of U2OS using CL488-28055

    IF Staining of U2OS using CL488-28055

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using CoraLite® Plus 488 NKCC1,SLC12A2 antibody (CL488-28055) at dilution of 1:200.

CoraLite® Plus 488-conjugated NKCC1,SLC12A2 Polyclonal antibody

NKCC1,SLC12A2 Polyclonal Antibody for IF/ICC
Cat No. CL488-28055

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC

别名

NKCC1, SLC12A2, Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2, BSC2, BSC

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inU2OS cells
For other applications, we recommend the unconjugated version of this antibody, 28055-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-28055 targets NKCC1,SLC12A2 in IF/ICC applications and shows reactivity with human samples.

经测试应用 IF/ICC Application Description
经测试反应性 human
免疫原 NKCC1,SLC12A2 fusion protein Ag27895 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 solute carrier family 12 (sodium/potassium/chloride transporters), member 2
别名 NKCC1, SLC12A2, Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2, BSC2, BSC
计算分子量 131 kDa
观测分子量160 kDa
GenBank蛋白编号BC033003
基因名称 NKCC1/SLC12A2
Gene ID (NCBI) 6558
RRIDAB_3672820
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP55011
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The Na-K-Cl cotransporter sodium-potassium-chloride cotransporter 1 (NKCC1), encoded by the SLC12A2 gene, is an intensively studied member of the CCC family. NKCC1 is a membrane protein and plays fundamental roles in regulating trans-epithelial ion movement, cell volume, chloride homeostasis and neuronal excitability. NKCC1 assembles into a dimer, with the first ten transmembrane (TM) helices harboring the transport core and TM11-TM12 helices lining the dimer interface(PMID: 32081947). The observed molecular weight of NKCC1 is about 140 kDa, glycosylated NKCC1 is detected a band at ∼170 kDa (PMID: 22723696).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 NKCC1,SLC12A2 antibody CL488-28055Download protocol
Standard Protocols
Click here to view our Standard Protocols