• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

CoraLite® Plus 488-conjugated NKCC1,SLC12A2 Polyclonal antibody

NKCC1,SLC12A2 Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-28055

Print datasheet

Synonyms

NKCC1, SLC12A2, Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2, BSC2, BSC

验证数据展示

  • IF Staining of U2OS using CL488-28055

    IF Staining of U2OS using CL488-28055

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using CoraLite® Plus 488 NKCC1,SLC12A2 antibody (CL488-28055) at dilution of 1:200.

经过测试的应用

Positive IF/ICC detected inU2OS cells
For other applications, we recommend the unconjugated version of this antibody, 28055-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-28055 targets NKCC1,SLC12A2 in IF/ICC applications and shows reactivity with human samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human
Immunogen NKCC1,SLC12A2 fusion protein Ag27895 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name solute carrier family 12 (sodium/potassium/chloride transporters), member 2
Synonyms NKCC1, SLC12A2, Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2, BSC2, BSC
Calculated Molecular Weight 131 kDa
Observed Molecular Weight160 kDa
GenBank Accession NumberBC033003
Gene Symbol NKCC1/SLC12A2
Gene ID (NCBI) 6558
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP55011
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The Na-K-Cl cotransporter sodium-potassium-chloride cotransporter 1 (NKCC1), encoded by the SLC12A2 gene, is an intensively studied member of the CCC family. NKCC1 is a membrane protein and plays fundamental roles in regulating trans-epithelial ion movement, cell volume, chloride homeostasis and neuronal excitability. NKCC1 assembles into a dimer, with the first ten transmembrane (TM) helices harboring the transport core and TM11-TM12 helices lining the dimer interface(PMID: 32081947). The observed molecular weight of NKCC1 is about 140 kDa, glycosylated NKCC1 is detected a band at ∼170 kDa (PMID: 22723696).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 NKCC1,SLC12A2 antibody CL488-28055Download protocol
Standard Protocols
Click here to view our Standard Protocols