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验证数据展示

  • WB analysis of mouse kidney using 16699-1-AP (same clone as 16699-1-PBS)

    WB analysis of mouse kidney using 16699-1-AP (same clone as 16699-1-PBS)

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16699-1-AP (NKD2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16699-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 16699-1-AP (same clone as 16699-1-PBS)

    WB analysis of HEK-293 using 16699-1-AP (same clone as 16699-1-PBS)

    WB result of NKD2 antibody (16699-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-NKD2 transfected HEK-293 cells. This data was developed using the same antibody clone with 16699-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

NKD2 Polyclonal antibody, PBS Only

NKD2 Polyclonal Antibody for WB, Indirect ELISA
Cat No. 16699-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, Indirect ELISA

别名

Naked-2, Naked2, Naked 2, hNkd2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

16699-1-PBS targets NKD2 in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 NKD2 fusion protein Ag10094 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 naked cuticle homolog 2 (Drosophila)
别名 Naked-2, Naked2, Naked 2, hNkd2
计算分子量 50 kDa
观测分子量 59 kDa
GenBank蛋白编号BC012176
基因名称 NKD2
Gene ID (NCBI) 85409
RRIDAB_2151468
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ969F2
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Naked2 (protein naked cuticle homolog 2), encoded by NKD2 gene, was identified as a cell autonomous antagonist of the canonical Wnt signaling pathway. Naked2 acts as a cargo recognition and targeting protein to ensure proper delivery and fusion of TGF-alpha-containing vesicles to a distinct region at the basolateral surface of polarized epithelial cells (PMID: 17553928). EGFR-independent action of TGF-alpha protects Naked2 from proteasomal degradation, thus ensuring its delivery to the basolateral surface (PMID: 18757723). Myristoylated Naked2 antagonizes Wnt-beta-catenin activity possibly by degrading Dishevelled-1 at the plasma membrane (PMID: 20177058).