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  • Featured Product
  • KD/KO Validated

NKRF Monoclonal antibody

NKRF Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat, Mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1H10C8

Cat no : 68230-1-Ig

Print datasheet

Synonyms

ITBA4, NF kappa B repressing factor, NFKB repressing factor, NKRF, NRF, Protein ITBA4, Transcription factor NRF

验证数据展示

  • WB analysis using 68230-1-Ig

    WB analysis using 68230-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68230-1-Ig (NKRF antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • WB analysis of HEK-293 using 68230-1-Ig

    WB analysis of HEK-293 using 68230-1-Ig

    WB result of NKRF antibody (68230-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NKRF transfected HEK-293 cells.

  • WB analysis using 68230-1-Ig

    WB analysis using 68230-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68230-1-Ig (NKRF antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

经过测试的应用

Positive WB detected inLNCaP cells, Jurkat cells, HEK-293 cells, MCF-7 cells, HeLa cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68230-1-Ig targets NKRF in WB, ELISA applications and shows reactivity with Human, Rat, Mouse samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human, Rat, Mouse
Immunogen NKRF fusion protein Ag6374 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name NFKB repressing factor
Synonyms ITBA4, NF kappa B repressing factor, NFKB repressing factor, NKRF, NRF, Protein ITBA4, Transcription factor NRF
Calculated Molecular Weight 78 kDa
Observed Molecular Weight100 kDa
GenBank Accession NumberBC068514
Gene Symbol NKRF
Gene ID (NCBI) 55922
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO15226
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NKRF, also named as Protein ITBA4 or Transcription factor NRF, is a 690 amino acid protein, which contains one G-patch domain and one R3H domain. NKRF is Widely and constitutively expressed, with lower level in colon, peripheral blood lymphocytes, lung and kidney. NKRF interacts with a specific negative regulatory element (NRE) 5'-AATTCCTCTGA-3' to mediate transcriptional repression of certain NK-kappa-B responsive genes. NKRF is involved in the constitutive silencing of the IFN-β promoter, independently of the virus-induced signals, and in the inhibition of the basal and cytokine-induced iNOS promoter activity.

实验方案

Product Specific Protocols
WB protocol for NKRF antibody 68230-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols