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验证数据展示

  • WB analysis using 68230-1-Ig

    WB analysis using 68230-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68230-1-Ig (NKRF antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • WB analysis of HEK-293 using 68230-1-Ig

    WB analysis of HEK-293 using 68230-1-Ig

    WB result of NKRF antibody (68230-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NKRF transfected HEK-293 cells.

  • WB analysis using 68230-1-Ig

    WB analysis using 68230-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68230-1-Ig (NKRF antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • Featured Product
  • KD/KO验证

NKRF Monoclonal antibody

NKRF Monoclonal Antibody for WB, ELISA
Cat No. 68230-1-Ig

产品说明书

CloneNo. 1H10C8

宿主/亚型

Mouse / IgG1

种属反应性

Human, Rat, Mouse

应用

WB, ELISA

别名

ITBA4, NF kappa B repressing factor, NFKB repressing factor, NKRF, NRF, Protein ITBA4, Transcription factor NRF

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inLNCaP cells, Jurkat cells, HEK-293 cells, MCF-7 cells, HeLa cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68230-1-Ig targets NKRF in WB, ELISA applications and shows reactivity with Human, Rat, Mouse samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human, Rat, Mouse
免疫原 NKRF fusion protein Ag6374 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 NFKB repressing factor
别名 ITBA4, NF kappa B repressing factor, NFKB repressing factor, NKRF, NRF, Protein ITBA4, Transcription factor NRF
计算分子量 78 kDa
观测分子量100 kDa
GenBank蛋白编号BC068514
基因名称 NKRF
Gene ID (NCBI) 55922
RRIDAB_2935317
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDO15226
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NKRF, also named as Protein ITBA4 or Transcription factor NRF, is a 690 amino acid protein, which contains one G-patch domain and one R3H domain. NKRF is Widely and constitutively expressed, with lower level in colon, peripheral blood lymphocytes, lung and kidney. NKRF interacts with a specific negative regulatory element (NRE) 5'-AATTCCTCTGA-3' to mediate transcriptional repression of certain NK-kappa-B responsive genes. NKRF is involved in the constitutive silencing of the IFN-β promoter, independently of the virus-induced signals, and in the inhibition of the basal and cytokine-induced iNOS promoter activity.

实验方案

Product Specific Protocols
WB protocol for NKRF antibody 68230-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols