• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 68230-1-Ig (same clone as 68230-1-PBS)

    WB analysis using 68230-1-Ig (same clone as 68230-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68230-1-Ig (NKRF antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 68230-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 68230-1-Ig (same clone as 68230-1-PBS)

    WB analysis of HEK-293 using 68230-1-Ig (same clone as 68230-1-PBS)

    WB result of NKRF antibody (68230-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NKRF transfected HEK-293 cells. This data was developed using the same antibody clone with 68230-1-PBS in a different storage buffer formulation.

  • WB analysis using 68230-1-Ig (same clone as 68230-1-PBS)

    WB analysis using 68230-1-Ig (same clone as 68230-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68230-1-Ig (NKRF antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 68230-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

NKRF Monoclonal antibody, PBS Only

NKRF Monoclonal Antibody for WB, Indirect ELISA
Cat No. 68230-1-PBS

产品说明书

CloneNo. 1H10C8

宿主/亚型

Mouse / IgG1

种属反应性

Human, Rat, Mouse

应用

WB, Indirect ELISA

别名

ITBA4, NF kappa B repressing factor, NFKB repressing factor, NKRF, NRF, Protein ITBA4, Transcription factor NRF

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

产品信息

68230-1-PBS targets NKRF in WB, Indirect ELISA applications and shows reactivity with Human, Rat, Mouse samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 Human, Rat, Mouse
免疫原 NKRF fusion protein Ag6374 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 NFKB repressing factor
别名 ITBA4, NF kappa B repressing factor, NFKB repressing factor, NKRF, NRF, Protein ITBA4, Transcription factor NRF
计算分子量 78 kDa
观测分子量100 kDa
GenBank蛋白编号BC068514
基因名称 NKRF
Gene ID (NCBI) 55922
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDO15226
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NKRF, also named as Protein ITBA4 or Transcription factor NRF, is a 690 amino acid protein, which contains one G-patch domain and one R3H domain. NKRF is Widely and constitutively expressed, with lower level in colon, peripheral blood lymphocytes, lung and kidney. NKRF interacts with a specific negative regulatory element (NRE) 5'-AATTCCTCTGA-3' to mediate transcriptional repression of certain NK-kappa-B responsive genes. NKRF is involved in the constitutive silencing of the IFN-β promoter, independently of the virus-induced signals, and in the inhibition of the basal and cytokine-induced iNOS promoter activity.