验证数据展示
经过测试的应用
| Positive IF/ICC detected in | THP-1 cells |
For other applications, we recommend the unconjugated version of this antibody, 83384-2-PBS
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-83384-2 targets NLRC5 in IF/ICC applications and shows reactivity with human samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | human |
| 免疫原 | NLRC5 fusion protein Ag34508 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | NLR family, CARD domain containing 5 |
| 别名 | |
| 计算分子量 | 204 kDa |
| GenBank蛋白编号 | BC063566 |
| 基因名称 | NLRC5 |
| Gene ID (NCBI) | 84166 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q86WI3 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The nucleotide-binding domain leucine-rich repeat containing (NLR) family of proteins is mainly involved in microbial pathogen recognition, inflammatory responses, and cell death. NLRC5, the largest member of the NLR family, is currently receiving an increasing level of attention. NLRC5 plays a role in cytokine response and antiviral immunity through its inhibition of NF-kappa-B activation and negative regulation of type I interferon signaling pathways.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 NLRC5 antibody CL488-83384-2 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |